Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V., Hans-Knöll-Institut., D-07745 Jena, Germany.
Int J Syst Evol Microbiol. 2015 Jan;65(Pt 1):195-200. doi: 10.1099/ijs.0.070375-0. Epub 2014 Oct 15.
A yellow, Gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain JM-1(T)) was isolated from the rhizosphere of a field-grown Zea mays plant in Auburn, AL, USA. 16S rRNA gene sequence analysis of strain JM-1(T) showed high sequence similarity to the type strains of Novosphingobium capsulatum (98.9%), Novosphingobium aromaticivorans (97.4%), Novosphingobium subterraneum (97.3%) and Novosphingobium taihuense (97.1%); sequence similarities to all other type strains of species of the genus Novosphingobium were below 97.0%. DNA-DNA hybridizations of strain JM-1(T) and N. capsulatum DSM 30196(T), N. aromaticivorans SMCC F199(T) and N. subterraneum SMCC B0478(T) showed low similarity values of 33% (reciprocal: 21%), 14% (reciprocal 16%) and 36% (reciprocal 38%), respectively. Ubiquinone Q-10 was detected as the major respiratory quinone. The predominant fatty acid was C18:1ω7c (71.0%) and the typical 2-hydroxy fatty acid C14:0 2-OH (11.7%) was detected. The polar lipid profile contained the diagnostic lipids diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and phosphatidylcholine. Characterization by 16S rRNA gene sequence analysis, physiological parameters, pigment analysis, and ubiquinone, polar lipid and fatty acid composition revealed that strain JM-1(T) represents a novel species of the genus Novosphingobium. For this species we propose the name Novosphingobium rhizosphaerae sp. nov. with the type strain JM-1(T) ( = LMG 28479(T) =CCM 8547(T)).
一株黄色、革兰氏阴性、杆状、不产芽孢的细菌（JM-1(T) 菌株）从美国阿拉巴马州奥本市田间生长的玉米根际中分离得到。JM-1(T) 菌株的 16S rRNA 基因序列分析显示，其与鞘氨醇单胞菌属的模式菌株（98.9%）、芳香环烃单胞菌（97.4%）、地下鞘氨醇单胞菌（97.3%）和太湖水生鞘氨醇单胞菌（97.1%）的序列相似性最高；与鞘氨醇单胞菌属所有其他模式菌株的序列相似性均低于 97.0%。JM-1(T) 菌株与鞘氨醇单胞菌属的 N. capsulatum DSM 30196(T)、N. aromaticivorans SMCC F199(T) 和 N. subterraneum SMCC B0478(T) 的 DNA-DNA 杂交相似性值分别为 33%（相互为 21%）、14%（相互为 16%）和 36%（相互为 38%）。检测到的主要呼吸醌为泛醌 Q-10。主要脂肪酸为 C18:1ω7c（71.0%），并检测到典型的 2-羟基脂肪酸 C14:0 2-OH（11.7%）。极性脂类谱包含诊断性脂类二磷脂酰甘油、磷脂酰乙醇胺、神经酰胺糖脂和磷脂酰胆碱。基于 16S rRNA 基因序列分析、生理参数、色素分析以及泛醌、极性脂类和脂肪酸组成的特征，JM-1(T) 菌株代表了鞘氨醇单胞菌属的一个新种。我们建议将该菌株命名为根际鞘氨醇单胞菌 Novosphingobium rhizosphaerae sp. nov.，其模式菌株为 JM-1(T)（ = LMG 28479(T) =CCM 8547(T)）。
