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sp. nov.,具有降解柴油的能力,从受污染土壤中分离得到,并提议将其重新分类为 的后异名。

sp. nov., with the ability to degrade diesel oil, isolated from oil-contaminated soil and proposal to reclassify as a later heterotypic synonym of .

机构信息

Department of Environmental Engineering, College of Science and Technology, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, 30019, Republic of Korea.

Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do 16227, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004628.

Abstract

Two yellow-pigmented, non-motile, Gram-stain-negative, and rod-shaped bacteria, designated TW-4 and TNP-2 were obtained from oil-contaminated soil. Both strains degrade diesel oil, hydrolyse aesculin, DNA, Tween 40 and Tween 60. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain TW-4 formed a lineage within the family and clustered as members of the genus . The closest members of strain TW-4 were DSM 12447 (97.9 %, sequence similarity), KSS165-70 (97.8 %), T3-B9 (97.8 %), DSM 12444 (97.7 %), UCT-28 (97.7 %), and STM-24 (97.6 %). The sequence similarity for other members was ≤97.6 %. The genome of strain TW-4 was 4 683 467 bp long with 44 scaffolds and 4280 protein-coding genes. The sole respiratory quinone was Q-10. The major cellular fatty acids were summed feature 8 (C 7 and/or C 6), summed feature 3 (C 7 and/or C 6), C and C 2-OH. The major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylcholine (PC), phosphatidyl-n-methylethanolamine (PME) and sphingoglycolipid (SGL). The DNA G+C content of the type strain was 65.0 %. The average nucleotide identity (ANIu) and DNA-DNA hybridization (dDDH) relatedness values between strain TW-4 and closest members were below the threshold value for species delineation. Based on polyphasic taxonomic analyses, strain TW-4 represents novel species in the genus , for which the name sp. nov. is proposed. The type strain is TW-4 (=KACC 21628=NBRC 114364) and strain TNP-2 (=KACC 21629=NBRC 114365) represents an additional strain. Based on new data obtained in this study, it is also proposed to reclassify as a later heterotypic synonym of .

摘要

从受石油污染的土壤中分离得到两株黄色、非运动、革兰氏阴性、杆状的细菌,分别命名为 TW-4 和 TNP-2。两株菌均能降解柴油,水解马栗树皮素、DNA、吐温 40 和吐温 60。基于 16S rRNA 基因序列的系统发育分析表明,菌株 TW-4 形成了一个家族内的谱系,并聚类为属的成员。菌株 TW-4 与最接近的成员为 DSM 12447(97.9%,序列相似度)、KSS165-70(97.8%)、T3-B9(97.8%)、DSM 12444(97.7%)、UCT-28(97.7%)和 STM-24(97.6%)。与其他成员的序列相似度≤97.6%。菌株 TW-4 的基因组长 4683467bp,有 44 个支架和 4280 个蛋白编码基因。唯一的呼吸醌是 Q-10。主要的细胞脂肪酸为 C7 和/或 C6 的总和特征 8、C7 和/或 C6 的总和特征 3、C 和 C2-OH。主要的极性脂为磷脂酰乙醇胺(PE)、磷脂酰甘油(PG)、双磷脂酰甘油(DPG)、磷脂酰胆碱(PC)、磷酰甲乙醇胺(PME)和神经酰胺脂(SGL)。模式菌株的 DNA G+C 含量为 65.0%。菌株 TW-4 与最接近的成员之间的平均核苷酸同一性(ANIu)和 DNA-DNA 杂交(dDDH)相关性值低于种系划定的阈值。基于多相分类学分析,菌株 TW-4 代表属中的一个新种,建议命名为 sp. nov.。模式菌株为 TW-4(=KACC 21628=NBRC 114364),菌株 TNP-2(=KACC 21629=NBRC 114365)代表另一个菌株。基于本研究获得的新数据,还建议将 重新分类为 的一个后期异名。

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