Gore Bryan B, Soden Marta E, Zweifel Larry S
Department of Pharmacology, University of Washington, Seattle, Washington 98053, USA Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle, Washington 98053, USA.
Department of Pharmacology, University of Washington, Seattle, Washington 98053, USA Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle, Washington 98053, USA
Learn Mem. 2014 Oct 15;21(11):575-9. doi: 10.1101/lm.036079.114. Print 2014 Nov.
Dopamine is broadly implicated in fear-related processes, yet we know very little about signaling dynamics in these neurons during active fear conditioning. We describe the direct imaging of calcium signals of dopamine neurons during Pavlovian fear conditioning using fiber-optic confocal microscopy coupled with the genetically encoded calcium indicator GCaMP3. We observed calcium transients in a subset of dopamine neurons to an unconditioned fear stimulus on the first day of Pavlovian fear conditioning. On the second day, calcium transients occurred in response to conditioned and unconditioned stimuli. These results demonstrate plasticity in dopamine neuron calcium signals and the occurrence of activity-dependent processes in these neurons during fear conditioning.
多巴胺广泛参与与恐惧相关的过程，但我们对主动恐惧条件反射过程中这些神经元的信号动力学了解甚少。我们使用光纤共聚焦显微镜结合基因编码钙指示剂GCaMP3，描述了巴甫洛夫恐惧条件反射过程中多巴胺能神经元钙信号的直接成像。在巴甫洛夫恐惧条件反射的第一天，我们观察到一部分多巴胺能神经元对无条件恐惧刺激出现钙瞬变。在第二天，对条件刺激和无条件刺激均出现钙瞬变。这些结果证明了多巴胺能神经元钙信号的可塑性以及在恐惧条件反射过程中这些神经元中发生的活动依赖过程。
