miR-140的下调通过靶向食管癌中的Slug诱导上皮-间质转化并促进侵袭。
Down-regulation of miR-140 induces EMT and promotes invasion by targeting Slug in esophageal cancer.
作者信息
Li Weiling, Jiang Guojun, Zhou Jianping, Wang Hongmin, Gong Zegang, Zhang Zheng, Min Ke, Zhu Hongdi, Tan Yongfei
机构信息
Department of Obstetrics and Gynecology, Affiliated Yixing Hospital of Jiangsu University, Yixing, People's Republic of China.
出版信息
Cell Physiol Biochem. 2014;34(5):1466-76. doi: 10.1159/000366351. Epub 2014 Oct 8.
BACKGROUND/AIMS: MicroRNAs (miRNAs) are reported to regulate cell invasion and functions by interfering with the translation of target mRNAs, but the role of miRNAs in esophageal cancer (EC) remains unclear.
METHODS
RT-PCR and Western blot were used to detect the expression of miRNAs and candidate genes in EC samples (n=89). miR-140 mimics and inhibitor were tansfected in human TE-1 and Eca-109 cells. The transwell assay was used to examine the cell invasive ability. The regulation mechanism was confirmed by luciferase reporter assay. The markers of EMT were detected by using Western blot.
RESULTS
miR-140 expression was decreased in the EC tissues compared with the corresponding adjacent tumor tissues. Low expression of miR-140 promotes cell invasion by using transwell assay, while the effect of miR-140 high expression is reverse. Slug, a target gene of miR-140, was examined by luciferase assay and Western blot.
CONCLUSIONS
miR-140 may regulate the cell invasion of EC via controlling Slug expression.
背景/目的：据报道，微小RNA（miRNA）通过干扰靶mRNA的翻译来调节细胞侵袭和功能，但miRNA在食管癌（EC）中的作用仍不清楚。
方法
采用逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测89例EC样本中miRNA和候选基因的表达。将miR-140模拟物和抑制剂转染到人TE-1和Eca-109细胞中。采用Transwell实验检测细胞侵袭能力。通过荧光素酶报告基因实验证实调控机制。采用蛋白质免疫印迹法检测上皮-间质转化（EMT）标志物。
结果
与相应的癌旁组织相比，EC组织中miR-140表达降低。Transwell实验显示，miR-140低表达促进细胞侵袭，而miR-140高表达则相反。通过荧光素酶实验和蛋白质免疫印迹法检测miR-140的靶基因Slug。
结论
miR-140可能通过控制Slug表达来调节EC细胞的侵袭。
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