Wang Jianwei, Zhu Jinhui, Dong Mingjun, Yu Hua, Dai Xiaoyu, Li Keqiang
Department of Surgical Oncology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.
Department of Anorectal Surgery, Ningbo Second Hospital, Ningbo, People's Republic of China.
Biotechnol Appl Biochem. 2015 Sep-Oct;62(5):621-7. doi: 10.1002/bab.1308. Epub 2015 Jan 14.
Protein phosphatase 5 (PP5) is a unique member of the protein phosphatases family that functions in multiple signaling pathways involved in DNA damage, cell cycle control, cell growth, and apoptosis. Recent evidence indicated that PP5 may play a role in cancer progression. In this study, we aimed to examine the biological effect of PP5 on cell growth and apoptosis in human colorectal cancer (CRC). We first knocked down PP5 expression in RKO cells via a short hairpin RNA containing lentivirus system. Then, methylthiazoletetrazolium assay, colony formation assay, and flow cytometry analysis were performed. The proliferation and colony formation ability of RKO cells were remarkably suppressed in PP5-silenced groups, as compared with control groups. Moreover, downregulation of PP5 resulted in a significant G0/G1 phase cell cycle arrest and an induction of apoptosis. In all, these results demonstrated the importance of PP5 in CRC cell growth, and it might be used as a potential therapeutic target for the treatment of CRC.
蛋白磷酸酶5（PP5）是蛋白磷酸酶家族中的一个独特成员，它在涉及DNA损伤、细胞周期调控、细胞生长和凋亡的多种信号通路中发挥作用。最近的证据表明，PP5可能在癌症进展中起作用。在本研究中，我们旨在研究PP5对人结直肠癌（CRC）细胞生长和凋亡的生物学效应。我们首先通过含短发夹RNA的慢病毒系统敲低RKO细胞中PP5的表达。然后，进行了甲基噻唑基四氮唑蓝测定、集落形成测定和流式细胞术分析。与对照组相比，PP5沉默组中RKO细胞的增殖和集落形成能力明显受到抑制。此外，PP5的下调导致显著的G0/G1期细胞周期阻滞并诱导凋亡。总之，这些结果证明了PP5在CRC细胞生长中的重要性，它可能作为治疗CRC的潜在治疗靶点。
