Han Kun, Gan Zhihua, Lin Shuchen, Hu Haiyan, Shen Zan, Min Daliu
Department of Medical Oncology, The Affiliated 6th People's Hospital of Shanghai Jiaotong University, Shanghai 200223, China.
Acta Biochim Pol. 2017;64(1):11-16. doi: 10.18388/abp.2014_951. Epub 2017 Jan 13.
Osteosarcoma is the most common primary malignant bone tumor in adolescents and young adults. However, the involvement of serine/threonine phosphatase type 5 (PP5) in osteosarcoma remains unclear. The aim of this study was to evaluate the functional role of PP5 in osteosarcoma cells. Firstly, we found that PP5 is widely expressed in several human osteosarcoma cell lines. Then we used lentivirus-delivered siRNA to silence PP5 expression in Saos-2 and U2OS cell lines. Knockdown of endogenous PP5 expression by shRNA-expressing lentivirus significantly decreased the viability and proliferation of the osteosarcoma cells. Moreover, FACS analysis showed that knockdown of PP5 expression induced a significant arrest in the G0/G1 phase of the cell cycle, which was associated with the inhibition of cell proliferation. Therefore, knockdown of PP5 is likely to provide a novel alternative to targeted therapy of osteosarcoma and deserves further investigation.
骨肉瘤是青少年和年轻成年人中最常见的原发性恶性骨肿瘤。然而，5型丝氨酸/苏氨酸磷酸酶（PP5）在骨肉瘤中的作用仍不清楚。本研究的目的是评估PP5在骨肉瘤细胞中的功能作用。首先，我们发现PP5在几种人骨肉瘤细胞系中广泛表达。然后我们使用慢病毒递送的小干扰RNA（siRNA）使Saos-2和U2OS细胞系中的PP5表达沉默。表达短发夹RNA（shRNA）的慢病毒敲低内源性PP5表达显著降低了骨肉瘤细胞的活力和增殖。此外，流式细胞术分析表明，PP5表达的敲低诱导细胞周期在G0/G1期显著停滞，这与细胞增殖的抑制有关。因此，敲低PP5可能为骨肉瘤的靶向治疗提供一种新的选择，值得进一步研究。
