Yamashita Y, Imai Y, Osawa T
Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Mol Immunol. 1989 Sep;26(9):905-13. doi: 10.1016/0161-5890(89)90147-8.
The lymph node (LN) T cells from autoimmune MRL/MpJ-lpr/lpr (lpr) mice and control MRL/MpJ-+/+ (+/+) mice were compared as to their cell surface lectin binding sites and the glycoproteins responsible for the lectin binding. T cells from enlarged lymph nodes of lpr mice were found to express more binding sites for lectins which are reactive to poly[N-acetyl-lactosamine]-type sugar chains than normal +/+ mouse lymph node T cells. Furthermore, we found that high mol. wt (180,000-220,000) glycoproteins on lpr T cells were strongly stained with these poly [N-acetyl-lactosamine]-binding lectins on Western-blotting. These glycoproteins were found to belong to the CD45 family on immunoprecipitation and absorption with monoclonal anti-CD45 antibody. Thus, aberrant expression of high mol. wt CD45 (CD45R) antigens on lpr T cells may contribute greatly to the strong reaction of these cells with poly[N-acetyl-lactosamine]-binding lectins. We also found that poly[N-acetyl-lactosamine]-type sugar chains are more abundant on B cells than on lpr T cells, and that the molecular weights and the carbohydrate moieties of CD45R antigens on lpr T cells are different from those of CD45R antigens on +/+ spleen B cells.
对自身免疫性MRL/MpJ-lpr/lpr（lpr）小鼠和对照MRL/MpJ-+/+（+/+）小鼠的淋巴结（LN）T细胞的细胞表面凝集素结合位点以及负责凝集素结合的糖蛋白进行了比较。发现lpr小鼠肿大淋巴结中的T细胞比正常+/+小鼠淋巴结T细胞表达更多与聚[N-乙酰乳糖胺]型糖链反应的凝集素结合位点。此外，我们发现在蛋白质印迹法中，lpr T细胞上的高分子量（180,000 - 220,000）糖蛋白被这些聚[N-乙酰乳糖胺]结合凝集素强烈染色。经单克隆抗CD45抗体免疫沉淀和吸附后发现，这些糖蛋白属于CD45家族。因此，lpr T细胞上高分子量CD45（CD45R）抗原的异常表达可能极大地促成了这些细胞与聚[N-乙酰乳糖胺]结合凝集素的强烈反应。我们还发现，聚[N-乙酰乳糖胺]型糖链在B细胞上比在lpr T细胞上更丰富，并且lpr T细胞上CD45R抗原的分子量和碳水化合物部分与+/+脾B细胞上的CD45R抗原不同。
