Imai Y, Yamashita Y, Osawa T
Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Mol Immunol. 1988 May;25(5):419-28. doi: 10.1016/0161-5890(88)90161-7.
Lymph node (LN) T cells from autoimmune MRL/MpJ-lpr/lpr (lpr) mice and control MRL/MpJ-+/+ (+/+) mice were compared as to their cell surface lectin-binding sites and glycosyltransferase activities. T cells from enlarged LN of lpr mice expressed a higher amount of binding sites for lectins reactive to mucin-type sugar chains than normal +/+ mouse T cells. Correspondingly, glycosyltransferase activities involved in the biosynthesis of mucin-type sugar chains were higher in lpr mouse T cells than in +/+ T cells. The activities of UDP-N-acetylgalactosamine (GalNAc):polypeptide GalNAc transferase and UDP-galactose (Gal):asialo bovine submaxillary mucin (BSM) Gal transferase were found to be elevated. The activity of UDP-Gal:asialo-agalacto transferrin Gal transferase, which is involved in the biosynthesis of complex type sugar chains, was also increased in lpr mice but to a smaller extent than the mucin-type Gal transferase activities. An abnormality in sialyltransferase activity was also found in lpr T cells.
对自身免疫性MRL/MpJ-lpr/lpr（lpr）小鼠和对照MRL/MpJ-+/+（+/+）小鼠的淋巴结（LN）T细胞的细胞表面凝集素结合位点和糖基转移酶活性进行了比较。与正常+/+小鼠T细胞相比，lpr小鼠肿大淋巴结中的T细胞对粘蛋白型糖链有反应的凝集素表达出更高数量的结合位点。相应地，lpr小鼠T细胞中参与粘蛋白型糖链生物合成的糖基转移酶活性高于+/+ T细胞。发现UDP-N-乙酰半乳糖胺（GalNAc）：多肽GalNAc转移酶和UDP-半乳糖（Gal）：去唾液酸牛下颌粘蛋白（BSM）Gal转移酶的活性升高。参与复合型糖链生物合成的UDP-Gal：去唾液酸-去半乳糖转铁蛋白Gal转移酶的活性在lpr小鼠中也有所增加，但增幅小于粘蛋白型Gal转移酶活性。在lpr T细胞中还发现了唾液酸转移酶活性异常。
