作为感染性细菌人工染色体克隆的细胞培养减毒豚鼠巨细胞病毒的全基因组序列。
Complete genome sequence of cell culture-attenuated Guinea pig cytomegalovirus cloned as an infectious bacterial artificial chromosome.
作者信息
Yang Dongmei, Alam Zohaib, Cui Xiaohong, Chen Michael, Sherrod Carly J, McVoy Michael A, Schleiss Mark R, Dittmer Dirk P
机构信息
University of North Carolina School of Medicine, Lineberger Comprehensive Cancer Center and Department of Microbiology and Immunology, Chapel Hill, North Carolina, USA.
Virginia Commonwealth University School of Medicine, Department of Pediatrics, Richmond, Virginia, USA.
出版信息
Genome Announc. 2014 Oct 16;2(5):e00928-14. doi: 10.1128/genomeA.00928-14.
Abstract
The complete genome sequence of attenuated guinea pig cytomegalovirus cloned as bacterial artificial chromosome N13R10 was determined. Comparison to pathogenic salivary gland-derived virus revealed 13 differences, 1 of which disrupted overlapping open reading frames encoding GP129 and GP130. Attenuation of N13R10 may arise from an inability to express GP129 and/or GP130.
摘要
测定了作为细菌人工染色体N13R10克隆的减毒豚鼠巨细胞病毒的全基因组序列。与致病性唾液腺来源病毒的比较揭示了13个差异，其中1个差异破坏了编码GP129和GP130的重叠开放阅读框。N13R10的减毒可能源于无法表达GP129和/或GP130。
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