结直肠癌肺转移瘤组织中的自噬活性：一项初步研究。
Autophagy activity in pulmonary metastatic tumor tissues from colorectal cancer: a pilot study.
作者信息
Kim Young Sam, Shin Ju Hye, Bae Mi Kyung, Lee Chang Young, Kim Dae Joon, Chung Kyung Young, Lee Jin Gu
机构信息
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
Department of Thoracic and Cardiovascular Surgery, Yonsei University College of Medicine, Seoul, Korea.
出版信息
Yonsei Med J. 2014 Nov;55(6):1484-8. doi: 10.3349/ymj.2014.55.6.1484.
PURPOSE
Autophagy has been reported to be involved in treatment failure in tumor. We aimed to evaluate autophagy activity in tumor tissue and compare them between the recurrence and non-recurrence groups.
MATERIALS AND METHODS
We analyzed expressions of autophagy-related proteins in tumor tissues which were obtained from pulmonary metastases of colorectal cancer patients by Western blot. We also analyzed autophagosomes by transmission electron microscopy.
RESULTS
Tumor tissues from recurrence group showed increased levels of LC3B-II, decreased levels of p62/SQSTM1, and also a marked accumulation of autophagosomes compared with tissues from non-recurrence group.
CONCLUSION
The present study suggests that autophagy may be associated with treatment failure of metastatic colorectal cancer.
目的
据报道自噬与肿瘤治疗失败有关。我们旨在评估肿瘤组织中的自噬活性，并比较复发组和非复发组之间的自噬活性。
材料与方法
我们通过蛋白质免疫印迹法分析了从结直肠癌患者肺转移灶获取的肿瘤组织中自噬相关蛋白的表达。我们还通过透射电子显微镜分析了自噬体。
结果
与非复发组的组织相比，复发组的肿瘤组织显示LC3B-II水平升高，p62/SQSTM1水平降低，并且自噬体明显积累。
结论
本研究表明自噬可能与转移性结直肠癌的治疗失败有关。
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