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自噬相关蛋白 10 作为溃疡性结肠炎生物标志物的潜力研究。

A study on the potential role of autophagy-related protein 10 as a biomarker for ulcerative colitis.

机构信息

Biochemistry Division, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Clinical Pathology Division, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

出版信息

Physiol Rep. 2021 Apr;9(7):e14825. doi: 10.14814/phy2.14825.

DOI:10.14814/phy2.14825
PMID:33904657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8077160/
Abstract

PURPOSE

Ulcerative colitis (UC) is a lifelong disease with unclear etiology and increasing prevalence worldwide. Autophagy has been reported to play roles in the pathogenesis and progression of UC. Here, we aimed to analyze the expression of autophagy related protein 10 (ATG10) and its regulator, micro-RNA (miR) 519a, in UC patients.

METHODS

The level of ATG10 in the serum, stool, and colon biopsies from 15 UC patients and 30 non-UC healthy individuals (HC) group was measured by ELISA. Also, the blood level of miR-519a was investigated by quantitative real-time PCR.

RESULTS

We found 13.63 ng/ml versus 0.99 ng/ml, 11.01 ng/ml versus 1.11 ng/ml and 6.41 ng/ml versus 3.21 ng/ml of ATG10 in the stool, colon tissue, and serum of UC and HC, respectively. There was no significant difference in the expression of miR-519a in the blood samples of UC and HC.

CONCLUSIONS

ATG10 might be a potential non-invasive diagnostic biomarker for UC.

摘要

目的

溃疡性结肠炎（UC）是一种病因不明且全球患病率不断增加的终身疾病。自噬在 UC 的发病机制和进展中起作用。在这里，我们旨在分析自噬相关蛋白 10（ATG10）及其调节剂 microRNA（miR）519a 在 UC 患者中的表达。

方法

通过 ELISA 检测 15 例 UC 患者和 30 例非 UC 健康个体（HC）组血清、粪便和结肠活检中 ATG10 的水平。还通过定量实时 PCR 检测 miR-519a 的血液水平。

结果

我们发现 UC 和 HC 组的粪便、结肠组织和血清中的 ATG10 分别为 13.63ng/ml 与 0.99ng/ml、11.01ng/ml 与 1.11ng/ml 和 6.41ng/ml 与 3.21ng/ml。UC 和 HC 患者血液样本中 miR-519a 的表达无显著差异。

结论

ATG10 可能是 UC 的一种潜在非侵入性诊断生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/8077160/9b5c3965a4f3/PHY2-9-e14825-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/8077160/70924a17afa3/PHY2-9-e14825-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/8077160/1a8f6633dc19/PHY2-9-e14825-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/8077160/9b5c3965a4f3/PHY2-9-e14825-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/8077160/70924a17afa3/PHY2-9-e14825-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/8077160/1a8f6633dc19/PHY2-9-e14825-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4951/8077160/9b5c3965a4f3/PHY2-9-e14825-g002.jpg

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