Akinrinmade Joseph Fadeyemi, Akinrinde Stephen Akinleye, Odejobi Atinuke, Oyagbemi Adetokunbo Ademola
J Complement Integr Med. 2015 Mar;12(1):23-32. doi: 10.1515/jcim-2014-0034.
Chromolena odorata is a tropical species of flowering shrub in the family Asteraceae, leaves of it have been reported to be widely used as herbal remedy for the treatment of various ailments. It is particularly reported to be useful in the healing of wounds.
We investigated the possibility of amelioration of intestinal ischemia-reperfusion (IR) injury in rats treated with methanolic extract of C. odorata (MECO). Wistar albino rats were divided randomly into five groups of six animals each as control, IR-treated, IR+200 mg/kg MECO, IR+400 mg/kg MECO, and IR+200 mg/kg vitamin C. Pre-treatment with MECO or vitamin C was for 7 days.
The contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) were significantly reduced by MECO and vitamin C, while there were significant enhancements of the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), as well as the content of reduced glutathione (GSH) in pre-treated rats compared to IR-treated rats. Glutathione S-transferase (GST) activity was not significantly affected in all the groups. Histopathological examination of small intestinal mucosa revealed significant attenuation of intestinal pathology in animals pre-treated with MECO, while IR injury produced severe villi erosion, necrosis, and inflammatory cell infiltrations.
The present study highlights the antioxidant activities of MECO and its ability to inhibit inflammatory cell infiltration as mechanisms involved in its protection against IR injury in the intestine of rats, an effect that was largely comparable to that of vitamin C.
香丝草是菊科一种热带开花灌木，据报道其叶子被广泛用作草药治疗各种疾病。特别报道称其对伤口愈合有用。
我们研究了用香丝草甲醇提取物（MECO）处理的大鼠改善肠道缺血再灌注（IR）损伤的可能性。将Wistar白化大鼠随机分为五组，每组六只动物，分别为对照组、IR处理组、IR + 200 mg/kg MECO组、IR + 400 mg/kg MECO组和IR + 200 mg/kg维生素C组。用MECO或维生素C预处理7天。
与IR处理组大鼠相比，MECO和维生素C显著降低了过氧化氢（H2O2）和丙二醛（MDA）的含量，同时预处理大鼠的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）、过氧化氢酶（CAT）活性以及还原型谷胱甘肽（GSH）含量显著增强。所有组中谷胱甘肽S - 转移酶（GST）活性均未受到显著影响。小肠黏膜的组织病理学检查显示，用MECO预处理的动物肠道病理显著减轻，而IR损伤导致严重的绒毛侵蚀、坏死和炎症细胞浸润。
本研究强调了MECO的抗氧化活性及其抑制炎症细胞浸润的能力，这些是其保护大鼠肠道免受IR损伤的机制，其效果与维生素C大致相当。
