叶片甲醇提取物对大鼠缺血再灌注损伤引起的急性肾损伤的肾保护作用。
Nephroprotective effect of methanol extract of leaves on acute kidney injury induced by ischemia-reperfusion in rats.
机构信息
Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Ibadan, Nigeria.
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, University of Ibadan, Nigeria.
出版信息
Afr Health Sci. 2020 Sep;20(3):1382-1396. doi: 10.4314/ahs.v20i3.44.
BACKGROUND
is known to exhibit protection against oxidative damage due to its rich content of compounds with antioxidant activity. This study investigated the protective effect of the methanol extract of (MO) in a rat model of renal ischemia-reperfusion (IR) injury.
METHODS
Forty two wistar rats were randomly assigned to six groups of seven rats each, as follows: A, control group; B, sham-operated group; C, IR group; D, IR + low dose (200 mg/kg) MO; E, IR + high dose (400 mg/kg) MO and F, IR + Vitamin C (200 mg/kg). Unilateral ischaemia was induced by occluding the left renal artery for 45 minutes followed by reperfusion up to 24 hours.
RESULTS
significantly (p<0.05) ameliorated IR-induced increases in malondialdehyde (MDA), protein carbonyls (PC) and advanced oxidation protein products (AOPP), while also decreasing serum BUN and Creatinine levels. Moreover, the low dose of MO caused reductions in renal NO and HO levels, while increasing renal GPx and GST activities. Histopathology revealed marked improvement of tissue alterations induced by IR with both doses of MO.
CONCLUSION
Overall, the methanol extract of effectively attenuated the deleterious effects of renal IR via alleviation of tissue oxidative stress.
背景
由于其富含具有抗氧化活性的化合物，因此被认为具有对抗氧化损伤的保护作用。本研究探讨了 （MO）甲醇提取物在肾缺血再灌注（IR）损伤大鼠模型中的保护作用。
方法
42 只 Wistar 大鼠随机分为 6 组，每组 7 只，如下：A、对照组；B、假手术组；C、IR 组；D、IR+低剂量（200mg/kg）MO 组；E、IR+高剂量（400mg/kg）MO 组和 F、IR+维生素 C（200mg/kg）组。通过阻断左肾动脉 45 分钟诱导单侧缺血，然后再灌注 24 小时。
结果
MO 显著（p<0.05）改善了 IR 诱导的丙二醛（MDA）、蛋白质羰基（PC）和高级氧化蛋白产物（AOPP）的增加，同时降低了血清 BUN 和肌酐水平。此外，低剂量的 MO 降低了肾 NO 和 HO 水平，同时增加了肾 GPx 和 GST 活性。组织病理学显示，两种剂量的 MO 均明显改善了 IR 引起的组织改变。
结论
总之， 甲醇提取物通过减轻组织氧化应激有效减轻了肾 IR 的有害影响。
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