病理性透明质酸基质中重链修饰和TSG-6活性分析
Analysis of the heavy-chain modification and TSG-6 activity in pathological hyaluronan matrices.
作者信息
Lauer Mark E, Loftis Jacqueline, de la Motte Carol, Hascall Vincent C
机构信息
Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH, 44195, USA,
出版信息
Methods Mol Biol. 2015;1229:543-8. doi: 10.1007/978-1-4939-1714-3_42.
Abstract
During inflammation and developmental processes, heavy chains (HCs) from inter-α-inhibitor (IαI) are covalently transferred to hyaluronan (HA) via the enzyme tumor-necrosis-factor-stimulated-gene 6 (TSG-6) to form a HC-HA complex. In this manuscript, we describe a gel-based assay to detect HC-HA and TSG-6 activity in tissues.
摘要
在炎症和发育过程中，来自α-抑制因子（IαI）的重链（HCs）通过肿瘤坏死因子刺激基因6（TSG-6）酶共价转移到透明质酸（HA）上，形成HC-HA复合物。在本论文中，我们描述了一种基于凝胶的检测方法，用于检测组织中的HC-HA和TSG-6活性。
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