在TRPM7镁离子通道mRNA的5'前导序列中鉴定出一个对镁离子敏感的开放阅读框。
Identification of a Mg2+-sensitive ORF in the 5'-leader of TRPM7 magnesium channel mRNA.
作者信息
Nikonorova Inna A, Kornakov Nikolay V, Dmitriev Sergey E, Vassilenko Konstantin S, Ryazanov Alexey G
机构信息
Department of Cellular and Molecular Pharmacology, Rutgers Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
Department of Cellular and Molecular Pharmacology, Rutgers Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia.
出版信息
Nucleic Acids Res. 2014 Nov 10;42(20):12779-88. doi: 10.1093/nar/gku951. Epub 2014 Oct 17.
TRPM7 is an essential and ubiquitous channel-kinase regulating cellular influx of Mg2+. Although TRPM7 mRNA is highly abundant, very small amount of the protein is detected in cells, suggesting post-transcriptional regulation of trpm7 gene expression. We found that TRPM7 mRNA 5'-leader contains two evolutionarily conserved upstream open reading frames that act together to drastically inhibit translation of the TRPM7 reading frame at high magnesium levels and ensure its optimal translation at low magnesium levels, when the activity of the channel-kinase is most required. The study provides the first example of magnesium channel synthesis being controlled by Mg2+ in higher eukaryotes.
瞬时受体电位阳离子通道亚家族M成员7（TRPM7）是一种调节细胞镁离子内流的必需且普遍存在的通道激酶。尽管TRPM7信使核糖核酸（mRNA）含量非常丰富，但在细胞中检测到的该蛋白量却非常少，这表明TRPM7基因表达存在转录后调控。我们发现，TRPM7 mRNA的5'前导序列包含两个进化上保守的上游开放阅读框，它们共同作用，在高镁水平下大幅抑制TRPM7阅读框的翻译，并在低镁水平下确保其最佳翻译，而此时通道激酶的活性最为需要。该研究提供了高等真核生物中镁离子通道合成受镁离子控制的首个实例。
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