Jockusch H, Füchtbauer E M, Füchtbauer A, Leger J J, Leger J, Maldonado C A, Forssmann W G
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7325-9. doi: 10.1073/pnas.83.19.7325.
Tissue fragments of newborn rat atria were transplanted under the dorsal skin or into the bed of the anterior tibial muscle of nude mice. After 5-11 weeks, the grafts, which had reorganized into beating atrium-like structures, were analyzed and compared to ventricular tissue transplanted the same way. As revealed by monoclonal antibodies against alpha- and beta-type myosin heavy chains, atrial grafts retained a typical pattern of myosin expression distinct from that of ventricular grafts. The majority of ectopic atrial myocytes contained specific atrial granules in which cardiodilatin-immunoreactive material has been localized. Specific granules and cardiodilatin immunoreactivity were not found in myocytes of ventricular grafts. We conclude that the long-term maintenance of isomyosin expression and of the myoendocrine function of atrial tissue is largely independent of the anatomical environment.
将新生大鼠心房的组织碎片移植到裸鼠的背部皮肤下或胫骨前肌床中。5 - 11周后，对重组为跳动的心房样结构的移植物进行分析，并与以相同方式移植的心室组织进行比较。抗α和β型肌球蛋白重链的单克隆抗体显示，心房移植物保留了与心室移植物不同的典型肌球蛋白表达模式。大多数异位心房肌细胞含有特定的心房颗粒，其中已定位了心钠素免疫反应性物质。在心室移植物的肌细胞中未发现特定颗粒和心钠素免疫反应性。我们得出结论，心房组织的异肌球蛋白表达和心肌内分泌功能的长期维持在很大程度上独立于解剖学环境。
