Moses R L, Delcarpio J B
Cell Tissue Res. 1983;228(3):489-96. doi: 10.1007/BF00211470.
Cardiac muscle cells from 3-day-old rat neonates were cultured for periods of 2 to 56 days. In order to facilitate ultrastructural studies on the organization of the sarcoplasmic reticulum, the cells were prepared for transmission electron microscopy according to a regimen including postfixation in reduced osmium ferrocyanide. The nonjunctional sarcoplasmic reticulum (NJSR) was organized as a loose, fenestrated sleeve around the exterior of bundles of myofilaments and was particularly prominent at the level of the Z line. The only recognizable junctional elements of the sarcoplasmic reticulum were in a peripheral location. Reduced osmium ferrocyanide was also useful in distinguishing intermediate (10 nm) filaments, since it understained Z substance, which often obscured these structures. Intermediate filaments were arranged both at the Z line and the intercalated disc, in parallel strands, approximately at right angles to the myofilaments.
对3日龄大鼠新生儿的心肌细胞进行了2至56天的培养。为了便于对肌浆网的组织结构进行超微结构研究，根据包括在还原锇铁氰化物中进行后固定的方案，制备用于透射电子显微镜检查的细胞。非连接肌浆网（NJSR）组织成围绕肌丝束外部的松散、有窗孔的套筒，并且在Z线水平特别突出。肌浆网唯一可识别的连接元件位于周边位置。还原锇铁氰化物在区分中间（10纳米）丝方面也很有用，因为它对通常会掩盖这些结构的Z物质染色不足。中间丝在Z线和闰盘处都有排列，呈平行束状，大致与肌丝成直角。
