Lin Aijun, Zhang Xuhong, Yang Xiaojin
Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China,
Ecotoxicology. 2014 Dec;23(10):2053-61. doi: 10.1007/s10646-014-1368-6. Epub 2014 Oct 19.
A pot culture experiment was carried out to investigate the roles of Glomus mosseae in Cu and Pb acquisition by upland rice (Oryza sativa L.) and the interactions between Cu and Pb. The soil was treated with three Cu levels (0, 100 and 200 mg kg(-1)) and three Pb levels (0, 300, and 600 mg kg(-1)). All treatments were designed with (+M) or without (-M) G. mosseae inoculation in a randomized block design. The addition of Cu and Pb significantly decreased root mycorrhizal colonization. Compared with -M, +M significantly increased root biomass in almost all treatments, and also significantly increased shoot biomass in the Pb(0)Cu(200), Pb(300)Cu(0), and all Pb(600) treatments. AM fungi enhanced plant Cu acquisition, but decreased plant Cu concentrations with all Cu plus Pb treatments, except for shoot in the Cu(200)Pb(600) treatment. Irrespective of Cu and Pb levels, +M plants had higher Pb uptakes than -M plants, but had lower root Pb and higher shoot Pb concentrations than those of -M plants. Another interpretation for the higher shoot Pb concentration in +M plants relied on Cu-Pb interactions. The study provided further evidences for the protective effects of AM fungi on upland rice against Cu and Pb contamination, and uncovered the phenomenon that Cu addition could promote Pb uptake and Pb partitioning to shoot. The possible mechanisms by which AM fungi can alleviate the toxicity induced by Cu and Pb are also discussed.
进行了一项盆栽试验，以研究摩西球囊霉在旱稻（Oryza sativa L.）吸收铜和铅中的作用以及铜和铅之间的相互作用。土壤用三种铜水平（0、100和200 mg kg(-1)）和三种铅水平（0、300和600 mg kg(-1)）处理。所有处理均采用随机区组设计，分为接种摩西球囊霉（+M）和未接种（-M）两组。添加铜和铅显著降低了根系菌根定殖率。与-M相比，+M在几乎所有处理中均显著增加了根系生物量，并且在Pb(0)Cu(200)、Pb(300)Cu(0)和所有Pb(600)处理中也显著增加了地上部生物量。丛枝菌根真菌提高了植物对铜的吸收，但在所有铜加铅处理中，除了Cu(200)Pb(600)处理的地上部外，均降低了植物铜浓度。无论铜和铅水平如何，+M植株的铅吸收量均高于-M植株，但根系铅浓度低于-M植株，地上部铅浓度高于-M植株。+M植株地上部铅浓度较高的另一种解释依赖于铜-铅相互作用。该研究为丛枝菌根真菌对旱稻抵御铜和铅污染的保护作用提供了进一步证据，并揭示了添加铜可促进铅吸收和铅向地上部分配的现象。还讨论了丛枝菌根真菌缓解铜和铅诱导毒性的可能机制。
