Gianoulakis C, Angelogianni P
Department of Psychiatry, McGill University, Verdun, Quebec, Canada.
Peptides. 1989 Sep-Oct;10(5):1049-54. doi: 10.1016/0196-9781(89)90189-7.
The objective of the present studies was to estimate the total content of beta-endorphin-like immunoreactivity (beta-EPLIR) and to characterize the beta-endorphin-like peptides in distinct regions of the spinal cord using gel filtration and reverse phase high performance liquid chromatography. The concentration of beta-EPLIR expressed as pg per mg tissue was similar in the four regions of the spinal cord. Sephadex G-75 chromatography demonstrated the presence, in all four regions of the spinal cord, of beta-endorphin (beta-EP) immunoreactive peptides eluting at the positions of standard beta-EP and beta-lipotropin (beta-LPH) peptides as well as a high molecular weight form eluting prior to beta-LPH. High performance liquid chromatography of the beta-EP-sized peptides indicated some differences in the relative proportions of the various beta-EP-sized peptides among the four regions of the spinal cord, which suggest a different origin of the beta-EP fibers terminating in different regions of the spinal cord as well as different physiological importance of the beta-endorphin peptides in the various spinal cord regions.
本研究的目的是使用凝胶过滤和反相高效液相色谱法估计β-内啡肽样免疫反应性（β-EPLIR）的总含量，并表征脊髓不同区域中的β-内啡肽样肽。以每毫克组织皮克表示的β-EPLIR浓度在脊髓的四个区域中相似。葡聚糖G-75色谱法表明，在脊髓的所有四个区域中，β-内啡肽（β-EP）免疫反应性肽在标准β-EP和β-促脂素（β-LPH）肽的位置洗脱，以及在β-LPH之前洗脱的高分子量形式。β-EP大小肽的高效液相色谱表明，脊髓四个区域中各种β-EP大小肽的相对比例存在一些差异，这表明终止于脊髓不同区域的β-EP纤维起源不同，以及β-内啡肽肽在脊髓不同区域的生理重要性不同。
