Yang Miling, Wang Lifeng, Yang Jinhua, Yang Guangying
Department of Pathology, the People's Hospital of Zhengzhou, Zhengzhou 450003, China.
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Zhonghua Bing Li Xue Za Zhi. 2014 Jul;43(7):473-7.
To investigate the expression of Versican in gastric carcinoma and its relationship with tumor angiogenesis.
Protein expression of Versican, vascular endothelial growth factor and CD34 was evaluated by immunohistochemistry (EliVision method) in 80 cases of gastric carcinoma and 30 samples of normal gastric tissue.
There were statistically significant differences in the expression of Versican, vascular endothelial growth factor and CD34 between gastric carcinoma and normal gastric tissue (P < 0.05). The expression of Versican was seen mainly in fibroblasts of the tumor and was correlated with tumor differentiation, clinical stage and lymph node metastasis (P < 0.05), whereas vascular endothelial growth factor was primarily seen in the cytoplasm of the tumor cells and correlated with tumor differentiation, clinical stage, Lauren classification and lymph node metastasis (P < 0.05). MVD was correlated with tumor differentiation, clinical stage, Lauren classification, depth of tumor invasion and lymph node metastasis (P < 0.05). In addition, positive correlation of Versican and VEGF protein expression was found in tumor cells (r = 0.467, P < 0.01).
The expression of both Versican and vascular endothelial growth factor is closely associated with tumor angiogenesis in gastric carcinoma.
探讨多功能蛋白聚糖（Versican）在胃癌中的表达及其与肿瘤血管生成的关系。
采用免疫组织化学法（EliVision法）检测80例胃癌组织及30例正常胃组织中Versican、血管内皮生长因子（VEGF）及CD34的蛋白表达。
胃癌组织与正常胃组织中Versican、VEGF及CD34的表达差异有统计学意义（P<0.05）。Versican主要表达于肿瘤的成纤维细胞，与肿瘤分化、临床分期及淋巴结转移相关（P<0.05）；而VEGF主要表达于肿瘤细胞的细胞质，与肿瘤分化、临床分期、Lauren分型及淋巴结转移相关（P<0.05）。微血管密度（MVD）与肿瘤分化、临床分期、Lauren分型、肿瘤浸润深度及淋巴结转移相关（P<0.05）。此外，肿瘤细胞中Versican与VEGF蛋白表达呈正相关（r=0.467，P<0.01）。
Versican和血管内皮生长因子的表达均与胃癌肿瘤血管生成密切相关。
