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不同溶剂对蔬菜残渣中酚类化合物提取的影响及其作为天然抗氧化剂源的评价。

Influence of different solvents in extraction of phenolic compounds from vegetable residues and their evaluation as natural sources of antioxidants.

机构信息

Central Institute of Post-Harvest Engineering and Technology, Ludhiana, 141 004 India.

Central Institute of Agricultural Engineering, Bhopal, 4620 038 India.

出版信息

J Food Sci Technol. 2014 Oct;51(10):2568-75. doi: 10.1007/s13197-012-0754-4. Epub 2012 Jun 19.

Abstract

Dried residues from four different vegetables, viz. pea pod (pp), cauliflower waste (CW), potato peel (PP) and tomato peel (TP) were extracted using four solvents i.e., hexane, chloroform, ethyl acetate and methanol. Among the four solvents, methanolic extracts showed the highest total phenolic content (TPC) for all the four vegetable residues. Methanolic extracts were evaluated for antioxidant activities using diphenylpicryl-hydrazyl (DPPH) and reducing power assay. Tomato peel extract showed highest phenolic content of 21.0 mg GAE/g-dw and 80.8 % DPPH free radical scavenging ability, whereas potato peel extract had a low phenolic content, and it also showed the least antioxidant activity among the residues examined in this study. Total phenolic content and DPPH free radical scavenging activity in pea pods and cauliflower waste were 13.6 mg GAE/g-dw and 72 % and 9.2 mg GAE/g-dw and 70.7 %, respectively. The coefficient of determination (r(2)) for correlation between TPC and reducing power, DPPH and TPC, DPPH and reducing power for all extracts was 0.85, 0.91and 0.87, respectively, suggesting an important role of phenolics in imparting antioxidant ability. Extracts from vegetables residues therefore represent a significant source of phenolic antioxidants for use as nutraceuticals or biopreservatives.

摘要

从四种不同的蔬菜(豌豆荚(pp)、菜花废料(CW)、土豆皮(PP)和西红柿皮(TP))中提取的干残余物分别使用四种溶剂(正己烷、氯仿、乙酸乙酯和甲醇)进行提取。在这四种溶剂中,甲醇提取物对所有四种蔬菜残渣的总酚含量(TPC)最高。使用二苯基苦基肼(DPPH)和还原力测定法评估甲醇提取物的抗氧化活性。西红柿皮提取物的酚含量最高,为 21.0 mg GAE/g-dw,DPPH 自由基清除能力为 80.8%,而土豆皮提取物的酚含量较低,在本研究中检测到的残渣中抗氧化活性最低。豌豆荚和菜花废料的总酚含量和 DPPH 自由基清除活性分别为 13.6 mg GAE/g-dw 和 72%,9.2 mg GAE/g-dw 和 70.7%。所有提取物的 TPC 与还原力、DPPH 与 TPC、DPPH 与还原力之间的决定系数(r(2))分别为 0.85、0.91 和 0.87,表明酚类物质在赋予抗氧化能力方面起着重要作用。因此,蔬菜残渣提取物是作为营养保健品或生物防腐剂使用的酚类抗氧化剂的重要来源。

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