采用正常细胞、神经胶质细胞和乳腺癌细胞体外细胞毒性检测评估 Fe(3)O(4)纳米粒子的生物相容性。
Biocompatibility of Fe(3)O(4) nanoparticles evaluated by in vitro cytotoxicity assays using normal, glia and breast cancer cells.
机构信息
Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
出版信息
Nanotechnology. 2010 Feb 19;21(7):75102. doi: 10.1088/0957-4484/21/7/075102. Epub 2010 Jan 21.
In order to reveal the biocompatibility of Fe(3)O(4) nanoparticles and bipolar surfactant tetramethylammonium 11-aminoundecanoate cytotoxicity tests were performed as a function of concentration from low (0.1 microg ml(-1)) to higher concentration (100 microg ml(-1)) using various human glia, human breast cancer and normal cell lines. Cytotoxicity tests for human glia (D54MG, G9T, SF126, U87, U251, U373), human breast cancer (MB157, SKBR3, T47D) and normal (H184B5F5/M10, WI-38, SVGp12) cell lines exhibited almost nontoxicity and reveal biocompatibility of Fe(3)O(4) nanoparticles in the concentration range of 0.1-10 microg ml(-1), while accountable cytotoxicity can be seen at 100 microg ml(-1). The results of our studies suggest that Fe(3)O(4) nanoparticles coated with bipolar surfactant tetramethylammonium 11-aminoundecanoate are biocompatible and promising for bio-applications such as drug delivery, magnetic resonance imaging and magnetic hyperthermia.
为了揭示 Fe(3)O(4) 纳米粒子的生物相容性，进行了双极性表面活性剂四甲基铵 11-氨基十一酸的细胞毒性试验，浓度范围从低浓度（0.1μg/ml）到高浓度（100μg/ml），使用了各种人类神经胶质细胞、人类乳腺癌和正常细胞系。对人类神经胶质细胞（D54MG、G9T、SF126、U87、U251、U373）、人类乳腺癌细胞（MB157、SKBR3、T47D）和正常细胞系（H184B5F5/M10、WI-38、SVGp12）的细胞毒性试验显示，几乎没有毒性，表明 Fe(3)O(4) 纳米粒子在 0.1-10μg/ml 的浓度范围内具有生物相容性，而在 100μg/ml 的浓度下可观察到可归因的细胞毒性。我们的研究结果表明，用双极性表面活性剂四甲基铵 11-氨基十一酸包覆的 Fe(3)O(4) 纳米粒子具有生物相容性，有望应用于药物输送、磁共振成像和磁热疗等生物应用。
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