Kumar Naveen, Kumar Pramod, Nayak Badagabettu Satheesha, Prasad Keerthana, Kudva Ranjini, Raghuveer Coimbatore Vasudevarao
Department of Anatomy, Melaka Manipal Medical College, Manipal University, Manipal Campus, Manipal, India.
Consultant Plastic Surgeon, King Abdulaziz Hospital, Al Jouf, Saudi Arabia.
Dermatol Res Pract. 2014;2014:251254. doi: 10.1155/2014/251254. Epub 2014 Sep 9.
Background. Histomorphic evaluation of dermal collagen and elastic fibres was analysed by image analysis technique. The quantification of dermal elements was performed in skin tissues, collected in horizontal and vertical directions from trunk region and discussed under the perspective of consequences of scar related complications. Materials and Method. Total number of 200 skin samples collected from 5 areas of trunk region were processed histologically and subjected to tissue-quant image analysis. Statistical analysis involving mean with SEM and paired t test by SPSS were employed to the percentage values obtained from image analysis. Result. Among the chosen 5 areas of trunk region, abdomen showed the statistically significant difference for both collagen and elastic content between horizontal and vertical orientations (P< 0.05), whereas upper back, presternal, and lateral chest areas showed significant difference (P< 0.05) only for collagen and groin only for elastic content. Conclusion. The differences in the distribution of dermal collagen and elastic fibres in 2 directions of the samples from the same areas might be attributed to final outcome of wound healing process by influencing the appearance and behaviour of scar related complications in the region of trunk.
背景。通过图像分析技术对真皮胶原纤维和弹性纤维进行组织形态学评估。在从躯干区域水平和垂直方向采集的皮肤组织中对真皮成分进行定量分析，并从瘢痕相关并发症后果的角度进行讨论。材料与方法。从躯干区域的5个部位收集了200个皮肤样本，进行组织学处理，并进行组织定量图像分析。使用SPSS对图像分析获得的百分比值进行涉及均值与标准误以及配对t检验的统计分析。结果。在所选的躯干区域5个部位中，腹部在水平和垂直方向的胶原和弹性成分含量上均显示出统计学显著差异（P<0.05），而上背部、胸骨前和侧胸部区域仅在胶原含量上显示出显著差异（P<0.05），腹股沟仅在弹性成分含量上显示出显著差异。结论。来自同一区域样本的两个方向上真皮胶原纤维和弹性纤维分布的差异可能归因于伤口愈合过程的最终结果，其通过影响躯干区域瘢痕相关并发症的外观和表现。
