Prabhu Gayathri S, Prasad Keerthana, K G Mohandas Rao, Rai Kiranmai S
Department of Anatomy, Melaka Manipal Medical College (Manipal campus), Manipal Academy of Higher Education, Karnataka, India.
Manipal School of Information Sciences, Manipal Academy of Higher Education, Karnataka, India.
Saudi J Biol Sci. 2021 Apr;28(4):2396-2407. doi: 10.1016/j.sjbs.2021.01.037. Epub 2021 Feb 4.
The aim of this study was to assess the efficacy of choline and DHA or exposure to environmental enrichment in obese adult and aging rats on alterations in body mass index, serum lipid profile and arterial wall changes, despite stopping high fat diet consumption and interventions during adulthood.
21 day old male Sprague Dawley rats were assigned as Experiment-1 & 2 - PND rats were divided into 4 groups with interventions for 7 months (n = 8/group). NC- Normal control fed normal chow diet; OB- Obese group, fed high fat diet; OB + CHO + DHA- fed high fat diet and oral supplementation of choline, DHA. OB + EE- fed high fat diet along with exposure to enriched environment .Experiment-2 had similar groups and interventions as experiment 1 but for next 5 months were fed normal chow diet without any interventions. Body mass index was assessed and blood was analyzed for serum lipid profile. Common Carotid Artery (CCA) was processed for Haematoxylin and eosin, Verhoff Vangeison stains. Images of tissue sections were analyzed and quantified using image J and tissue quant software.
In experiment.1, mean body mass index (p < 0.001), serum lipid profile (p < 0.01), thickness of tunica intima (p < 0.05), tunica media (p < 0.01) and percentage of collagen fibers (p < 0.01) of CCA were significantly increased in OB compared to NC. These were significantly attenuated in OB + CHO + DHA and OB + EE compared to OB. In experiment.2, mean body mass index (p < 0.01), serum lipid profile (p < 0.05) and thickness of tunica media of CCA (p < 0.01) were significantly increased in OB compared to NC. In OB + CHO + DHA and OB + EE, significant attenuation was observed in mean body mass index and mean thickness of tunica media compared to same in OB.
Adult obesity has negative impact on body mass index, serum lipid profile and arterial wall structure that persists through aging. Supplementation of choline and DHA or exposure to enriched environment during obesity attenuates these negative impacts through aging.
本研究的目的是评估在成年肥胖和衰老大鼠中，尽管在成年期停止了高脂肪饮食摄入和干预措施，但胆碱和二十二碳六烯酸（DHA）或环境富集暴露对体重指数、血清脂质谱和动脉壁变化的影响。
将21日龄雄性Sprague Dawley大鼠分为实验1和实验2 - 出生后第21天的大鼠分为4组，进行7个月的干预（每组n = 8）。NC - 正常对照组，喂食正常饲料；OB - 肥胖组，喂食高脂肪饮食；OB + CHO + DHA - 喂食高脂肪饮食并口服补充胆碱、DHA。OB + EE - 喂食高脂肪饮食并暴露于富集环境。实验2的分组和干预与实验1相似，但在接下来的5个月中喂食正常饲料且无任何干预。评估体重指数，并分析血液中的血清脂质谱。对颈总动脉（CCA）进行苏木精和伊红、Verhoff Vangeison染色处理。使用Image J和组织定量软件对组织切片图像进行分析和定量。
在实验1中，与NC组相比，OB组的平均体重指数（p < 0.001）、血清脂质谱（p < 0.01）、内膜厚度（p < 0.05）、中膜厚度（p < 0.01）和CCA胶原纤维百分比（p < 0.01）显著增加。与OB组相比，OB + CHO + DHA组和OB + EE组的这些指标显著降低。在实验2中，与NC组相比，OB组的平均体重指数（p < 0.01）、血清脂质谱（p < 0.05）和CCA中膜厚度（p < 0.01）显著增加。与OB组相比，OB + CHO + DHA组和OB + EE组的平均体重指数和中膜平均厚度显著降低。
成年肥胖对体重指数、血清脂质谱和动脉壁结构有负面影响，这种影响会持续到衰老。肥胖期间补充胆碱和DHA或暴露于富集环境可减轻衰老过程中的这些负面影响。
