Kumar Naveen, Kumar Pramod, Nayak Badagabettu Satheesha, Prasad Keerthana, Kudva Ranjini, Vasudevarao Raghuveer Coimbatore
Department of Anatomy, Melaka Manipal Medical College, Manipal Campus, Manipal University, Manipal 576104, India.
Department of Plastic Surgery, King Abdul Aziz Hospital, Sakaka, Al-Jouf 42421, Saudi Arabia.
Plast Surg Int. 2014;2014:364573. doi: 10.1155/2014/364573. Epub 2014 Dec 23.
Background. Clinically, scar related complications are observed to be dissimilar in different regions of the body. Unequal distribution of dermal collagen and elastic fibres in different orientations could be one of the multifocal causes of scar related complications, for which this evaluating study has been taken up. Materials and Method. 300 skin samples collected in horizontal and vertical orientations were studied histomorphometrically. This study involved image analysis of specially stained histological section using tissue-quant software. The outcome result was termed as quantitative fraction. From the result, various ratio values were also calculated for the ratio analysis. Results. The differences in the quantitative fraction of dermal elastic content between 2 directions were statistically significant at joint areas (shoulder joint, wrist, and ankle) (P < 0.001) but for collagen, significant difference was observed at shoulder joint and wrist only. Dermis of the forearm and thigh did not show any differences in their collagen content, but for elastic, thigh did show a significant difference while forearm had no change between 2 directions. Conclusion. Analysis of unequal content of dermal element in two directions under the perspective of wound healing consequences is subjective depending upon the anatomical position and functional status of the areas.
背景。临床上观察到，瘢痕相关并发症在身体的不同部位表现各异。不同方向的真皮胶原蛋白和弹性纤维分布不均可能是瘢痕相关并发症的多因素成因之一，为此开展了本评估研究。材料与方法。对300份水平和垂直方向采集的皮肤样本进行组织形态计量学研究。本研究涉及使用组织定量软件对特殊染色的组织切片进行图像分析。结果结果称为定量分数。根据结果，还计算了各种比值用于比值分析。结果。两个方向之间真皮弹性成分定量分数的差异在关节部位（肩关节、腕关节和踝关节）具有统计学意义（P < 0.001），但对于胶原蛋白，仅在肩关节和腕关节观察到显著差异。前臂和大腿的真皮胶原蛋白含量没有差异，但对于弹性纤维，大腿表现出显著差异，而前臂两个方向之间没有变化。结论。从伤口愈合后果的角度分析两个方向上真皮成分含量不均，取决于各区域的解剖位置和功能状态，具有主观性。
