Li G, Fraker P J
Zhongguo Yao Li Xue Bao. 1989 May;10(3):216-21.
The effects of methionine-enkephalin (Met-Enk) on mitogenic and mixed lymphocyte culture (MLC) proliferation of splenocytes from Zn-deficient, restricted and control mice were evaluated. The data from this experiment show that Met-Enk can suppress the responses of splenocyte from the 3 groups to concanavalin A (Con A), but less inhibition was observed in the Zn-deficient group. Met-Enk can also enhance the responses to pokeweed mitogen (PWM) and decrease the response to lipopolysaccharide (LPS) in all groups. Alteration of proliferative responses to Con A and PWM were reversible in the presence of naloxone 10 mumol/L indicating that the effect of Met-Enk on cellular proliferation was mediated by the opioid receptor. In the proliferation of MLC, the response of lymphocytes from Zn-deficient mice was increased in the absence of Met-Enk and Met-Enk can suppress this increased response. It is therefore concluded that Met-Enk can modify the pattern of mitogenic responses and the alteration in Con A and MLC responses can be influenced by zinc deficiency.
评估了甲硫氨酸脑啡肽（Met-Enk）对缺锌、限食和对照小鼠脾细胞有丝分裂原及混合淋巴细胞培养（MLC）增殖的影响。本实验数据表明，Met-Enk可抑制三组脾细胞对刀豆蛋白A（Con A）的反应，但在缺锌组中观察到的抑制作用较小。Met-Enk还可增强所有组对商陆有丝分裂原（PWM）的反应，并降低对脂多糖（LPS）的反应。在10 μmol/L纳洛酮存在的情况下，对Con A和PWM增殖反应的改变是可逆的，这表明Met-Enk对细胞增殖的作用是由阿片受体介导的。在MLC增殖中，缺锌小鼠淋巴细胞在无Met-Enk时反应增强，而Met-Enk可抑制这种增强的反应。因此得出结论，Met-Enk可改变有丝分裂原反应模式，且缺锌可影响Con A和MLC反应的改变。
