Dumri Kanchana, Hung Anh Dau
Department of Chemistry and Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Research and Development Institute/Department of Chemistry, Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Chiang Mai 50300, Thailand.
Enzyme Res. 2014;2014:389739. doi: 10.1155/2014/389739. Epub 2014 Sep 10.
Biodiesel production technology is competitive in terms of low cost and alternative source of energy which should be not only sustainable but also environmentally friendly. Designing of the lipase immobilization for biodiesel production has a remarkable impact and is still challenging. In this work, biodiesel production from soybean oil was enhanced and facilitated by using a novel biocatalyst consisting of commercial lipase (EC 3.1.1.3), silver nanoparticles, and polydopamine. Silver nanoparticles (AgNPs) were synthesized with a size range of 10-20 nm. Polydopamine (PD) was delivered by the self-polymerization of dopamine in 10 mM Tris-HCl pH 8.5 and simultaneously coated the AgNPs to form a PD/AgNPs complex. Lipase was immobilized on the PD/AgNPs complex surface via covalent bonds to form a tailor-made biocatalyst consisting of immobilized lipase/PD/AgNPs complex (LPA). The formation and morphology of each composition were characterized by UV-Vis spectroscopy and scanning electron microscope (SEM). Significantly, gas chromatography analysis showed a remarkable biodiesel production yield of 95% by using the LPA complex at 40(°)C for 6-hours reaction time, whereas the yield was 86% when using free lyophilized lipase. The LPA complex was apparently reusable after 7 batches and the latter conversion rate of soybean oil was decreased by only 27%.
生物柴油生产技术在低成本和替代能源方面具有竞争力，这种能源不仅应具备可持续性，还应环保。设计用于生物柴油生产的脂肪酶固定化方法具有显著影响，但仍具有挑战性。在这项工作中，通过使用一种由商业脂肪酶（EC 3.1.1.3）、银纳米颗粒和聚多巴胺组成的新型生物催化剂，提高并促进了从大豆油生产生物柴油的过程。合成的银纳米颗粒（AgNPs）尺寸范围为10 - 20纳米。聚多巴胺（PD）通过多巴胺在10 mM Tris - HCl pH 8.5中自聚合产生，并同时包覆AgNPs形成PD/AgNPs复合物。脂肪酶通过共价键固定在PD/AgNPs复合物表面，形成一种定制的生物催化剂，即固定化脂肪酶/PD/AgNPs复合物（LPA）。通过紫外可见光谱和扫描电子显微镜（SEM）对每种成分的形成和形态进行了表征。值得注意的是，气相色谱分析表明，在40℃反应6小时的条件下，使用LPA复合物时生物柴油的产率显著达到95%，而使用游离冻干脂肪酶时产率为86%。LPA复合物在7批次后显然可重复使用，且大豆油的后续转化率仅降低了27%。
