Wu Q X
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989 Aug;11(4):293-7.
In this article, we first report the development of a new test for detecting specific circulating immune complexes (SCIC) in the sera of leprosy patients: This test was named monoclonal antibody specific binding assay (McAb/SBA). We screened for SCIC (PGLI-IgG, PGLI-IgM) in 200 serum batches from 140 leprosy patients, 20 tuberculosis patients and 40 normal controls, and compared the McAb/SBA with PGL1/ELISA. The results indicated that: 1) McAb/SBA was highly sensitive (90%) and specific (95%). Its Youden's Index was 90. Except for its specificity of 95%, the sensitivity (85%) and Youden's Index (85) of the PGL1/ELISA test were lower than those of McAB/SBA; 2) The positive rate (34/40) in paucibacillary patients using McAb/SBA was higher than that (28/40) in PGL1/ELISA; 3) The increase and decline of MOD values in McAb/SBA were associated with BI. McAb/SBA is a new method for detecting SCIC (PGL1-IgG, PGL1-IgM) in the sera of leprosy patients, and it is more sensitive than PGL1-ELISA.
在本文中，我们首次报告了一种用于检测麻风病患者血清中特异性循环免疫复合物（SCIC）的新检测方法：该检测方法被命名为单克隆抗体特异性结合测定法（McAb/SBA）。我们对140例麻风病患者、20例结核病患者和40例正常对照的200份血清样本进行了SCIC（PGLI-IgG、PGLI-IgM）筛查，并将McAb/SBA与PGL1/ELISA进行了比较。结果表明：1）McAb/SBA具有高敏感性（90%）和高特异性（95%）。其约登指数为90。除其特异性为95%外，PGL1/ELISA检测的敏感性（85%）和约登指数（85）均低于McAB/SBA；2）使用McAb/SBA的少菌型患者阳性率（34/40）高于PGL1/ELISA（28/40）；3）McAb/SBA中MOD值的升高和下降与BI相关。McAb/SBA是一种检测麻风病患者血清中SCIC（PGL1-IgG、PGL1-IgM）的新方法，且比PGL1-ELISA更敏感。
