Tosti-Croce C, Thibault G, Garcia R, Schiller P W, Cantin M
Clinical Research Institute of Montreal, Quebec.
Clin Invest Med. 1989 Dec;12(6):381-5.
The effects of ANF (99-126) and its active analog, [3-mercaptopropionic 105]-ANF (105-126), on diuresis and natriuresis were assessed in the rat after intramuscular (i.m.) or subcutaneous (s.c.) injection. Water and sodium excretion increased significantly after i.m. or s.c. administration of either peptide in the first 20-min urine collection. ANF (99-126) was more active i.m. than its analog, while they both produced similar diuresis and natriuresis when they were administered s.c. The rapid and significant increase of plasma IR-ANF levels, detected by radioimmunoassay and analyzed by high performance liquid chromatography after i.m. or s.c. injection of these peptides, indicates very rapid passage into the blood circulation without degradation. These data demonstrate that either the i.m. or s.c. route can be used to administer ANF or its analog.
在大鼠中，通过肌肉注射（i.m.）或皮下注射（s.c.）评估了心钠素（ANF，99-126）及其活性类似物[3-巯基丙酸105]-ANF（105-126）对利尿和利钠的影响。在首次收集20分钟尿液时，肌肉注射或皮下注射任一肽后，水和钠排泄均显著增加。ANF（99-126）肌肉注射时比其类似物活性更强，而皮下注射时两者产生相似的利尿和利钠作用。通过放射免疫测定法检测并经高效液相色谱分析，在肌肉注射或皮下注射这些肽后，血浆免疫反应性心钠素（IR-ANF）水平迅速且显著升高，表明其能非常迅速地进入血液循环且未被降解。这些数据表明，肌肉注射或皮下注射途径均可用于给予ANF或其类似物。
