Gutkowska J, Horký K, Schiffrin E L, Thibault G, Garcia R, De Léan G A, Hamet P, Tremblay J, Anand-Srivastava M B, Januszewicz P
Fed Proc. 1986 Jun;45(7):2101-5.
A simple and sensitive radioimmunoassay was developed for measurement of immunoreactive atrial natriuretic factor (IR-ANF) in rat and human plasma and in rat atria. The two atria contain about 20 micrograms ANF per rat. The right atrium contained 2.5 times more ANF than did the left. Ether anesthesia and morphine markedly increased IR-ANF in rat plasma. The concentration of IR-ANF in plasma of clinically normal human subjects was 65.3 +/- 2.5 pg/ml. Paroxysmal tachycardia and rapid atrial pacing significantly increased IR-ANF in human plasma. Two- to seven-fold higher concentrations were found in coronary sinus blood than in the peripheral circulation. In the plasma of rats and humans, circulating ANF is probably a small-molecular-weight peptide. ANF acts on the adrenal and the pituitary. ANF inhibits aldosterone secretion from rat zona glomerulosa and steroid secretion by bovine adrenal zona glomerulosa and fasciculata. ANF stimulated the basal secretion of arginine vasopressin (AVP) in vitro and inhibited KCl-stimulated release of AVP.
已开发出一种简单且灵敏的放射免疫分析法，用于测定大鼠和人血浆以及大鼠心房中的免疫反应性心房利钠因子（IR-ANF）。每只大鼠的两个心房约含20微克ANF。右心房所含ANF比左心房多2.5倍。乙醚麻醉和吗啡可显著增加大鼠血浆中的IR-ANF。临床正常人类受试者血浆中IR-ANF的浓度为65.3±2.5皮克/毫升。阵发性心动过速和快速心房起搏可显著增加人血浆中的IR-ANF。在冠状窦血中发现的浓度比外周循环中的高2至7倍。在大鼠和人的血浆中，循环中的ANF可能是一种小分子量肽。ANF作用于肾上腺和垂体。ANF抑制大鼠球状带醛固酮分泌以及牛肾上腺球状带和束状带的类固醇分泌。ANF在体外刺激精氨酸加压素（AVP）的基础分泌，并抑制氯化钾刺激的AVP释放。
