外泌体中的抗原转移至树突状细胞，以此解释IgE免疫复合物所产生的免疫增强作用。
Antigen transfer from exosomes to dendritic cells as an explanation for the immune enhancement seen by IgE immune complexes.
作者信息
Martin Rebecca K, Brooks Keith B, Henningsson Frida, Heyman Birgitta, Conrad Daniel H
机构信息
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, United States of America.
Department of Medical Biochemistry and Microbiology, Biomedical Center, Uppsala University, Uppsala, Sweden.
出版信息
PLoS One. 2014 Oct 20;9(10):e110609. doi: 10.1371/journal.pone.0110609. eCollection 2014.
IgE antigen complexes induce increased specific T cell proliferation and increased specific IgG production. Immediately after immunization, CD23(+) B cells capture IgE antigen complexes, transport them to the spleen where, via unknown mechanisms, dendritic cells capture the antigen and present it to T cells. CD23, the low affinity IgE receptor, binds IgE antigen complexes and internalizes them. In this study, we show that these complexes are processed onto B-cell derived exosomes (bexosomes) in a CD23 dependent manner. The bexosomes carry CD23, IgE and MHC II and stimulate antigen specific T-cell proliferation in vitro. When IgE antigen complex stimulated bexosomes are incubated with dendritic cells, dendritic cells induce specific T-cell proliferation in vivo, similar to IgE antigen complexes. This suggests that bexosomes can provide the essential transfer mechanism for IgE antigen complexes from B cells to dendritic cells.
IgE抗原复合物可诱导特异性T细胞增殖增加以及特异性IgG产生增加。免疫后立即，CD23(+) B细胞捕获IgE抗原复合物，将其转运至脾脏，在脾脏中，树突状细胞通过未知机制捕获抗原并将其呈递给T细胞。CD23，即低亲和力IgE受体，结合IgE抗原复合物并将其内化。在本研究中，我们表明这些复合物以CD23依赖的方式被加工成B细胞来源的外泌体（bexosomes）。这些bexosomes携带CD23、IgE和MHC II，并在体外刺激抗原特异性T细胞增殖。当用IgE抗原复合物刺激的bexosomes与树突状细胞一起孵育时，树突状细胞在体内诱导特异性T细胞增殖，类似于IgE抗原复合物。这表明bexosomes可以为IgE抗原复合物从B细胞到树突状细胞提供必要的转运机制。
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