Selbie L A, Hayes G, Shine J
Garvan Institute of Medical Research, St. Vincent's Hospital, Sydney NSW, Australia.
DNA. 1989 Nov;8(9):683-9. doi: 10.1089/dna.1.1989.8.683.
The structural diversity of the human D2 dopamine receptor was examined at the nucleic acid level. Sequence analysis of receptor cDNA clones isolated from human brain and pituitary libraries and polymerase chain reaction (PCR) analysis of rat brain RNA and human genomic DNA demonstrate the presence of a predominant D2 subtype, D2A. The D2A subtype differs from the D2B subtype, previously described in rat brain RNA, in that an additional 29 amino acids are present in the putative third cytoplasmic domain, a region thought to be important for coupling to different G-proteins. The demonstration of intron sequences flanking the DNA encoding the 29-amino-acid insertion suggests that the generation of two distinct D2 dopamine receptor subtypes may arise from alternative splicing of a common genomic sequence.
在核酸水平上研究了人类D2多巴胺受体的结构多样性。从人脑和垂体文库中分离出的受体cDNA克隆的序列分析，以及大鼠脑RNA和人类基因组DNA的聚合酶链反应（PCR）分析表明，存在一种主要的D2亚型，即D2A。D2A亚型与先前在大鼠脑RNA中描述的D2B亚型不同，在于在假定的第三个细胞质结构域中存在另外29个氨基酸，该区域被认为对于与不同的G蛋白偶联很重要。编码29个氨基酸插入片段的DNA侧翼内含子序列的证实表明，两种不同的D2多巴胺受体亚型的产生可能源于共同基因组序列的可变剪接。
