Stamm S, Zhang M Q, Marr T G, Helfman D M
Cold Spring Harbor Laboratory, NY 11724.
Nucleic Acids Res. 1994 May 11;22(9):1515-26. doi: 10.1093/nar/22.9.1515.
Alternative splicing is an important regulatory mechanism to create protein diversity. In order to elucidate possible regulatory elements common to neuron specific exons, we created and statistically analysed a database of exons that are alternatively spliced in neurons. The splice site comparison of alternatively and constitutively spliced exons reveals that some, but not all alternatively spliced exons have splice sites deviating from the consensus sequence, implying diverse patterns of regulation. The deviation from the consensus is most evident at the -3 position of the 3' splice site and the +4 and -3 position of the 5' splice site. The nucleotide composition of alternatively and constitutively spliced exons is different, with alternatively spliced exons being more AU rich. We performed overlapping k-tuple analysis to identify common motifs. We found that alternatively and constitutively spliced exons differ in the frequency of several trinucleotides that cannot be explained by the amino acid composition and may be important for splicing regulation.
可变剪接是产生蛋白质多样性的一种重要调控机制。为了阐明神经元特异性外显子共有的可能调控元件，我们创建并统计分析了一个在神经元中发生可变剪接的外显子数据库。可变剪接外显子与组成型剪接外显子的剪接位点比较表明，一些（但不是所有）可变剪接外显子的剪接位点偏离共有序列，这意味着存在多种调控模式。在3'剪接位点的-3位置以及5'剪接位点的+4和-3位置，与共有序列的偏差最为明显。可变剪接外显子和组成型剪接外显子的核苷酸组成不同，可变剪接外显子富含AU。我们进行了重叠k元组分析以识别共同基序。我们发现，可变剪接外显子和组成型剪接外显子在几种三核苷酸的频率上存在差异，这些差异无法用氨基酸组成来解释，可能对剪接调控很重要。
