Egbadzor Kenneth F, Ofori Kwadwo, Yeboah Martin, Aboagye Lawrence M, Opoku-Agyeman Michael O, Danquah Eric Y, Offei Samuel K
West Africa Centre for Crop Improvement, University of Ghana, Legon, Accra Ghana ; CSIR - Plant Genetic Resources Research Institute, Bunso, Ghana.
Department of Crop Science, University of Ghana, Legon, Accra Ghana.
Springerplus. 2014 Sep 20;3:541. doi: 10.1186/2193-1801-3-541. eCollection 2014.
Single Nucleotide Polymorphism (SNP) markers were used in characterization of 113 cowpea accessions comprising of 108 from Ghana and 5 from abroad. Leaf tissues from plants cultivated at the University of Ghana were genotyped at KBioscience in the United Kingdom. Data was generated for 477 SNPs, out of which 458 revealed polymorphism. The results were used to analyze genetic dissimilarity among the accessions using Darwin 5 software. The markers discriminated among all of the cowpea accessions and the dissimilarity values which ranged from 0.006 to 0.63 were used for factorial plot. Unexpected high levels of heterozygosity were observed on some of the accessions. Accessions known to be closely related clustered together in a dendrogram drawn with WPGMA method. A maximum length sub-tree which comprised of 48 core accessions was constructed. The software package structure was used to separate accessions into three groups, and the programme correctly identified varieties that were known hybrids. The hybrids were those accessions with numerous heterozygous loci. The structure plot showed closely related accessions with similar genome patterns. The SNP markers were more efficient in discriminating among the cowpea germplasm than morphological, seed protein polymorphism and simple sequence repeat studies reported earlier on the same collection.
单核苷酸多态性（SNP）标记用于对113份豇豆种质进行特征分析，其中108份来自加纳，5份来自国外。在加纳大学种植的植株的叶片组织在英国的KBioscience进行基因分型。共生成了477个SNP的数据，其中458个显示出多态性。利用达尔文5软件，这些结果被用于分析种质间的遗传差异。这些标记区分了所有豇豆种质，范围从0.006到0.63的差异值被用于因子图分析。在一些种质中观察到了意外的高杂合度。已知亲缘关系较近的种质在用加权配对算术平均法（WPGMA）绘制的树状图中聚集在一起。构建了一个由48个核心种质组成的最大长度子树。使用软件包Structure将种质分为三组，该程序正确识别出了已知的杂交品种。这些杂交品种是那些具有众多杂合位点的种质。结构图谱显示亲缘关系较近的种质具有相似的基因组模式。与之前对同一批收集材料进行的形态学、种子蛋白多态性和简单序列重复研究相比，SNP标记在区分豇豆种质方面更有效。
