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芽殖酵母代谢组学的核磁共振分析：一种快速的样品制备方法。

NMR analysis of budding yeast metabolomics: a rapid method for sample preparation.

作者信息

Airoldi C, Tripodi F, Guzzi C, Nicastro R, Coccetti P

机构信息

Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy.

出版信息

Mol Biosyst. 2015 Feb;11(2):379-83. doi: 10.1039/c4mb00452c. Epub 2014 Oct 21.

DOI:10.1039/c4mb00452c
PMID:25333203
Abstract

Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by (1)H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.

摘要

在此，我们提出了一种优化方案，用于从酵母细胞中快速且可重复地提取细胞内代谢物，并通过¹H-NMR光谱对其进行代谢谱分析。通过比较不同生长阶段或不同遗传背景的细胞代谢组，验证了该方案的可靠性。

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