Callesen Henrik, Liu Ying, Pedersen Hanne S, Li Rong, Schmidt Mette
1 Department of Animal Science, Aarhus University , DK-8830, Tjele, Denmark .
Cell Reprogram. 2014 Dec;16(6):407-10. doi: 10.1089/cell.2014.0053. Epub 2014 Oct 21.
The low efficiency in obtaining piglets after production of cloned embryos was challenged in two steps-first by performing in vitro culture for 5-6 days after cloning to obtain later-stage embryos for more precise selection for transfer, and second by reducing the number of embryos transferred per recipient sow. The data set consisted of combined results from a 4-year period where cloning was performed to produce piglets that were transgenic for important human diseases. For this, different transgenes and cell types were used, and the cloning work was performed by several persons using oocytes from different pig breeds, but following a standardized and optimized protocol. Results showed that in vitro culture is possible with a relatively stable rate of transferable embryos around 41% and a pregnancy rate around 90%. Furthermore, a reduction from around 80 embryos to 40 embryos transferred per recipient was possible without changing the efficiency of around 14% (piglets born out of embryos transferred). It was concluded that this approach can increase the efficiency in obtaining piglets by means of in vitro culture and selection of high-quality embryos with subsequent transfer into more recipients. Such changes can also reduce the need for personnel, time, and material when working with this technology.
克隆胚胎生产后获得仔猪的效率低下问题分两步解决——首先，克隆后进行5至6天的体外培养，以获得后期胚胎，以便更精确地选择用于移植的胚胎；其次，减少每个受体母猪移植的胚胎数量。数据集包含4年期间的综合结果，在此期间进行克隆以生产针对重要人类疾病的转基因仔猪。为此，使用了不同的转基因和细胞类型，克隆工作由几个人进行，他们使用来自不同猪品种的卵母细胞，但遵循标准化和优化的方案。结果表明，体外培养是可行的，可移植胚胎的比例相对稳定在41%左右，妊娠率约为90%。此外，每个受体移植的胚胎数量可以从约80个减少到40个，而不改变约14%的效率（移植胚胎出生的仔猪）。得出的结论是，这种方法可以通过体外培养和选择高质量胚胎，随后移植到更多受体中来提高获得仔猪的效率。这些改变还可以减少使用该技术时对人员、时间和材料的需求。
