Nie Jun-Yu, Zhu Xiang-Xing, Xie Bing-Kun, Nong Su-Qun, Ma Qing-Yan, Xu Hui-Yan, Yang Xiao-Gan, Lu Yang-Qing, Lu Ke-Huan, Liao Yu-Ying, Lu Sheng-Sheng
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi High Education Key Laboratory for Animal Reproduction and Biotechnology, College of Animal Science and Technology, Guangxi University, Nanning, 530004, China.
Guangxi Key Laboratory of Livestock Genetic Improvement, Guangxi Institute of Animal Sciences, Nanning, 530001, China.
3 Biotech. 2016 Dec;6(2):218. doi: 10.1007/s13205-016-0525-4. Epub 2016 Oct 8.
Somatic cloning, also known as somatic cell nuclear transfer (SCNT), is a promising technology which has been expected to rapidly extend the population of elaborately selected breeding boars with superior production performance. Chinese Guike No. 1 pig breed is a novel swine specialized strain incorporated with the pedigree background of Duroc and Chinese Luchuan pig breeds, thus inherits an excellent production performance. The present study was conducted to establish somatic cloning procedures of adult breeding boars from the Chinese Guike No. 1 specialized strain. Ear skin fibroblasts were first isolated from a three-year-old Chinese Guike No. 1 breeding boar, and following that, used as donor cell to produce nuclear transfer embryos. Such cloned embryos showed full in vitro development and with the blastocyst formation rate of 18.4 % (37/201, three independent replicates). Finally, after transferring of 1187 nuclear transfer derived embryos to four surrogate recipients, six live piglets with normal health and development were produced. The overall cloning efficiency was 0.5 % and the clonal provenance of such SCNT derived piglets was confirmed by DNA microsatellite analysis. All of the cloned piglets were clinically healthy and had a normal weight at 1 month of age. Collectively, the first successful cloning of an adult Chinese Guike No. 1 breeding boar may lay the foundation for future improving the pig production industry.
体细胞核移植技术，也称为体细胞克隆，是一项很有前景的技术，有望快速扩大精心挑选的具有优良生产性能的种公猪群体。中国桂科1号猪是一个新的猪专门化品系，融合了杜洛克猪和中国陆川猪的系谱背景，因而具有优良的生产性能。本研究旨在建立中国桂科1号专门化品系成年种公猪的体细胞核移植程序。首先从一头3岁的中国桂科1号种公猪分离出耳部皮肤成纤维细胞，随后用作供体细胞来生产核移植胚胎。这些克隆胚胎在体外发育完全，囊胚形成率为18.4%（201枚胚胎中有37枚形成囊胚，3次独立重复）。最后，将1187枚核移植胚胎移植到4头代孕受体母猪体内，产出了6头健康发育正常的仔猪。总体克隆效率为0.5%，通过DNA微卫星分析确认了这些体细胞核移植仔猪的克隆来源。所有克隆仔猪临床健康，1月龄时体重正常。总之，首例成年中国桂科1号种公猪的成功克隆可能为未来改善养猪业奠定基础。
