Ruder Arne Mathias, Schmidt Michaela, Ludiro Alessia, Riva Marco A, Gass Peter
1] Department of Psychiatry and Psychotherapy, Central Institute of Mental Health, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany. [2].
Department of Pharmacological Sciences, Centre of Neuropharmacology, Università degli Studi di Milano, Milan, Italy.
Lab Anim (NY). 2014 Nov;43(11):405-9. doi: 10.1038/laban.591.
Carrying out invasive procedures in animals requires the administration of anesthesia. Xenon gas offers advantages as an anesthetic agent compared with other agents, such as its protection of the brain and heart from hypoxia-induced damage. The high cost of xenon gas has limited its use as an anesthetic in animal experiments, however. The authors designed and constructed simple boxes for the induction and maintenance of xenon gas and isoflurane anesthesia in small rodents in order to minimize the amount of xenon gas that is wasted. While using their anesthesia delivery system to anesthetize pregnant mice undergoing caesarean sections, they measured the respiratory rates of the anesthetized mice, the survival of the pups and the percentages of oxygen and carbon dioxide within the system to confirm the system's safety.
对动物进行侵入性操作需要使用麻醉剂。与其他麻醉剂相比，氙气作为一种麻醉剂具有优势，例如它能保护大脑和心脏免受缺氧诱导的损伤。然而，氙气的高成本限制了它在动物实验中作为麻醉剂的使用。作者设计并制作了简单的箱子，用于在小型啮齿动物中诱导和维持氙气和异氟烷麻醉，以尽量减少氙气的浪费量。在使用他们的麻醉输送系统麻醉接受剖腹产的怀孕小鼠时，他们测量了麻醉小鼠的呼吸频率、幼崽的存活率以及系统内氧气和二氧化碳的百分比，以确认该系统的安全性。
