Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Science, Capital Normal University, Beijing, 100048, China.
College of Agricultural and Biological Engineering (College of Tree Peony), Heze University, 2269 Daxue Road, Heze, 274015, Shandong, China.
BMC Genomics. 2022 May 14;23(1):369. doi: 10.1186/s12864-022-08599-1.
Drought stress is the most limiting factor for plant growth and crop production worldwide. As a major cereal crop, wheat is susceptible to drought. Thus, discovering and utilizing drought-tolerant gene resources from related species are highly important for improving wheat drought resistance. In this study, the drought tolerance of wheat Zhongmai 8601-Thinopyrum intermedium 7XL/7DS translocation line YW642 was estimated under drought stress, and then two-dimensional difference gel electrophoresis (2D-DIGE) based proteome analysis of the developing grains was performed to uncover the drought-resistant proteins.
The results showed that 7XL/7DS translocation possessed a better drought-tolerance compared to Zhongmai 8601. 2D-DIGE identified 146 differential accumulation protein (DAP) spots corresponding to 113 unique proteins during five grain developmental stages of YW642 under drought stress. Among them, 55 DAP spots corresponding to 48 unique proteins displayed an upregulated expression, which were mainly involved in stress/defense, energy metabolism, starch metabolism, protein metabolism/folding and transport. The cis-acting element analysis revealed that abundant stress-related elements were present in the promoter regions of the drought-responsive protein genes, which could play important roles in drought defense. RNA-seq and RT-qPCR analyses revealed that some regulated DAP genes also showed a high expression level in response to drought stress.
Our results indicated that Wheat-Th. intermedium 7XL/7DS translocation line carried abundant drought-resistant proteins that had potential application values for wheat drought tolerance improvement.
干旱胁迫是全球范围内植物生长和作物生产的最主要限制因素。作为主要的谷类作物，小麦易受干旱影响。因此，发现和利用相关物种的耐旱基因资源对于提高小麦的抗旱性非常重要。本研究在干旱胁迫下评估了小麦中麦 8601-中间偃麦草 7XL/7DS 易位系 YW642 的耐旱性，然后对发育籽粒进行基于二维差异凝胶电泳（2D-DIGE）的蛋白质组分析，以揭示耐旱蛋白。
结果表明，7XL/7DS 易位系比中麦 8601 具有更好的耐旱性。在干旱胁迫下，YW642 的五个籽粒发育阶段共鉴定到 146 个差异积累蛋白（DAP）点，对应 113 个独特蛋白。其中，55 个 DAP 点对应 48 个独特蛋白表达上调，主要涉及应激/防御、能量代谢、淀粉代谢、蛋白质代谢/折叠和转运。顺式作用元件分析表明，耐旱蛋白基因启动子区存在大量与应激相关的元件，可能在干旱防御中发挥重要作用。RNA-seq 和 RT-qPCR 分析表明，一些受调控的 DAP 基因对干旱胁迫也表现出高表达水平。
本研究结果表明，小麦-中间偃麦草 7XL/7DS 易位系携带丰富的耐旱蛋白，具有提高小麦耐旱性的潜在应用价值。
