Ma Yan-Zhi, Ke Shao-Ying
Zhong Yao Cai. 2014 May;37(5):736-40.
To identify tetraploid Isatis indigotica strains through morphology and flow cytometry.
The tissue culture seedlings of tetraploid Isatis indigotica were root-tip squashed and chromosome counted before rooted climatized and transplanted in field. The plants in field were taken as experimental materials. Macroscopic observation was applied to identify by form and structure; Free-hand section was used to observe the length, width and density of stomas; And flow cytometry was applied to identify the ploidy.
Compared with diploid plants, tetraploid plants had obvious changes in form and structure. The stomas from the tetraploid were notably longer, and the number of guard cells in chloroplasts was remarkably larger. The experiment materials were proved to be tetraploid by flow cytometry.
The materials are tetraploid plants. Macroscopic observation, the length of stoma and the number of guard cells in chloroplasts can be taken as aided identification for ploidy of mutagenesis materials. Meanwhile, flow cytometry can be applied to identify the ploidy of Isatis indigotica.
通过形态学和流式细胞术鉴定四倍体菘蓝品系。
四倍体菘蓝组培苗在生根驯化并移栽至田间前进行根尖压片和染色体计数。以田间植株作为实验材料，通过形态和结构进行宏观观察鉴定；徒手切片观察气孔长度、宽度和密度；采用流式细胞术鉴定倍性。
与二倍体植株相比，四倍体植株在形态和结构上有明显变化。四倍体的气孔明显更长，叶绿体中保卫细胞数量显著更多。通过流式细胞术证明实验材料为四倍体。
该材料为四倍体植株。宏观观察、气孔长度和叶绿体中保卫细胞数量可作为诱变材料倍性的辅助鉴定指标。同时，流式细胞术可用于鉴定菘蓝的倍性。
