Guerreiro-Tanomaru Juliane M, Pereira Kamila Figueiredo, Nascimento Camila Almeida, Bernardi Maria Inês Basso, Tanomaru-Filho Mario
Acta Odontol Latinoam. 2013;26(3):144-8.
The aim of this study was to evaluate the pH and antimicrobial activity of micro or nanoparticulate zinc oxide (ZnO) pastes with or without calcium hydroxide (CH). The following medications were evaluated: microparticulate ZnO + polyethylene glycol (PEG) 400; nanoparticulate ZnO + PEG 400; PEG 400; CH + microparticulate ZnO + PEG 400 and CH + nanoparticulate ZnO + PEG 400. The pH was assessed between 12 hours and 28 days, using a digital pH meter. The antimicrobial activity against Enterococcus faecalis (ATCC-9212), Candida albicans (ATCC-10231), Pseudomonas aeruginosa (ATCC-27853), Staphylococcus aureus (ATCC-6538) and Kocuria rhizophila (ATCC-9341) was determined in triplicate using agar diffusion test. The results were submitted to Kruskal-Wallis/Dunn and ANOVA/Tukey tests with 5% significance. The highest pH values were found for CH+ZnO, with higher values for nanoparticulate ZnO after 12 hours and 21 days (p < 0.05). CH+ZnO medication promoted higher growth inhibition against P. aeruginosa and lower against E. faecalis. Calcium hydroxide pastes have higher pH and antimicrobial activity when associated with either micro- or nanoparticulate zinc oxide.
本研究的目的是评估添加或不添加氢氧化钙（CH）的微米或纳米颗粒氧化锌（ZnO）糊剂的pH值和抗菌活性。评估了以下药物：微米颗粒ZnO+聚乙二醇（PEG）400；纳米颗粒ZnO+PEG 400；PEG 400；CH+微米颗粒ZnO+PEG 400以及CH+纳米颗粒ZnO+PEG 400。使用数字pH计在12小时至28天之间评估pH值。采用琼脂扩散试验一式三份测定对粪肠球菌（ATCC - 9212）、白色念珠菌（ATCC - 10231）、铜绿假单胞菌（ATCC - 27853）、金黄色葡萄球菌（ATCC - 6538）和嗜根考克氏菌（ATCC - 9341）的抗菌活性。结果采用Kruskal - Wallis/Dunn和ANOVA/Tukey检验，显著性水平为5%。CH+ZnO的pH值最高，纳米颗粒ZnO在12小时和21天后的值更高（p<0.05）。CH+ZnO药物对铜绿假单胞菌的生长抑制作用更强，对粪肠球菌的抑制作用较弱。当与微米或纳米颗粒氧化锌结合时，氢氧化钙糊剂具有更高的pH值和抗菌活性。
