Peng Ying, Clark Karl J, Campbell Jarryd M, Panetta Magdalena R, Guo Yi, Ekker Stephen C
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA Mayo Addiction Research Center, Mayo Clinic, Rochester, MN 55905, USA Center for Clinical and Translational Science, Mayo Clinic, Rochester, MN 55905, USA.
Development. 2014 Nov;141(21):4042-54. doi: 10.1242/dev.102186.
Recent advances in the targeted modification of complex eukaryotic genomes have unlocked a new era of genome engineering. From the pioneering work using zinc-finger nucleases (ZFNs), to the advent of the versatile and specific TALEN systems, and most recently the highly accessible CRISPR/Cas9 systems, we now possess an unprecedented ability to analyze developmental processes using sophisticated designer genetic tools. In this Review, we summarize the common approaches and applications of these still-evolving tools as they are being used in the most popular model developmental systems. Excitingly, these robust and simple genomic engineering tools also promise to revolutionize developmental studies using less well established experimental organisms.
复杂真核生物基因组靶向修饰的最新进展开启了基因组工程的新纪元。从使用锌指核酸酶（ZFNs）的开创性工作，到通用且特异的转录激活因子样效应物核酸酶（TALEN）系统的出现，以及最近高度易用的CRISPR/Cas9系统，我们现在拥有了前所未有的能力，可利用精密的设计基因工具来分析发育过程。在本综述中，我们总结了这些仍在不断发展的工具在最常用的模式发育系统中的常见方法和应用。令人兴奋的是，这些强大而简单的基因组工程工具也有望彻底改变对较不常用实验生物的发育研究。
