Gandhimathi Chinnasamy, Venugopal Jayarama Reddy, Bhaarathy Velmurugan, Ramakrishna Seeram, Kumar Srinivasan Dinesh
Cellular and Molecular Epigenetics Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.
Center for Nanofibers and Nanotechnology, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, Singapore.
Int J Nanomedicine. 2014 Oct 8;9:4709-22. doi: 10.2147/IJN.S65335. eCollection 2014.
Nanotechnology and tissue engineering have enabled engineering of nanostructured strategies to meet the current challenges in skin tissue regeneration. Electrospinning technology creates porous nanofibrous scaffolds to mimic extracellular matrix of the native tissues. The present study was performed to gain some insights into the applications of poly(l-lactic acid)-co-poly-(ε-caprolactone) (PLACL)/silk fibroin (SF)/vitamin E (VE)/curcumin (Cur) nanofibrous scaffolds and to assess their potential for being used as substrates for the culture of human dermal fibroblasts for skin tissue engineering. PLACL/SF/VE/Cur nanofibrous scaffolds were fabricated by electrospinning and characterized by fiber morphology, membrane porosity, wettability, mechanical strength, and chemical properties by Fourier transform infrared (FTIR) analysis. Human dermal fibroblasts were cultured on these scaffolds, and the cell scaffold interactions were analyzed by cell proliferation, cell morphology, secretion of collagen, expression of F-actin, and 5-chloromethylfluorescein diacetate (CMFDA) dye. The electrospun nanofiber diameter was obtained between 198±4 nm and 332±13 nm for PLACL, PLACL/SF, PLACL/SF/VE, and PLACL/SF/VE/Cur nanofibrous scaffolds. FTIR analysis showed the presence of the amide groups I, II, and III, and a porosity of up to 92% obtained on these nanofibrous scaffolds. The results showed that the fibroblast proliferation, cell morphology, F-actin, CMFDA dye expression, and secretion of collagen were significantly increased in PLACL/SF/VE/Cur when compared to PLACL nanofibrous scaffolds. The accessibility of human dermal fibroblasts cultured on PLACL/SF/VE/Cur nanofibrous scaffolds proved to be a potential scaffold for skin tissue regeneration.
纳米技术和组织工程学推动了纳米结构策略的工程化，以应对皮肤组织再生当前面临的挑战。静电纺丝技术可制造出多孔纳米纤维支架，以模拟天然组织的细胞外基质。开展本研究是为了深入了解聚（L-乳酸）-共聚（ε-己内酯）（PLACL）/丝素蛋白（SF）/维生素E（VE）/姜黄素（Cur）纳米纤维支架的应用，并评估其作为皮肤组织工程中人类真皮成纤维细胞培养底物的潜力。通过静电纺丝制备PLACL/SF/VE/Cur纳米纤维支架，并通过纤维形态、膜孔隙率、润湿性、机械强度以及傅里叶变换红外（FTIR）分析的化学性质对其进行表征。将人类真皮成纤维细胞培养在这些支架上，并通过细胞增殖、细胞形态、胶原蛋白分泌、F-肌动蛋白表达以及5-氯甲基荧光素二乙酸酯（CMFDA）染料来分析细胞与支架的相互作用。对于PLACL、PLACL/SF、PLACL/SF/VE和PLACL/SF/VE/Cur纳米纤维支架，静电纺纳米纤维直径在198±4纳米至332±13纳米之间。FTIR分析表明存在酰胺基团I、II和III，并且在这些纳米纤维支架上获得了高达92%的孔隙率。结果表明，与PLACL纳米纤维支架相比，PLACL/SF/VE/Cur中的成纤维细胞增殖、细胞形态、F-肌动蛋白、CMFDA染料表达以及胶原蛋白分泌均显著增加。在PLACL/SF/VE/Cur纳米纤维支架上培养的人类真皮成纤维细胞的可及性证明其是皮肤组织再生的潜在支架。
