微小RNA对TrkB可变转录本的调控
Regulation of a TrkB Alternative Transcript by microRNAs.
作者信息
Wong Jenny
机构信息
Illawarra Health and Medical Research Institute and School of Biological Sciences, University of Wollongong, Wollongong, N.S.W., Australia.
出版信息
Dement Geriatr Cogn Dis Extra. 2014 Sep 11;4(3):364-74. doi: 10.1159/000365917. eCollection 2014 Sep.
BACKGROUND/AIMS: Tropomyosin-related kinase B receptor (TrkB)-mediated signaling is vital for neuronal differentiation, survival, plasticity, and cognition. In this study, the focus was placed on TrkB-Shc, a neuron-specific transcript, to determine if microRNAs (miRNAs) play a role in TrkB-Shc regulation.
METHODS
A combination of bioinformatics and molecular gene expression analysis techniques was used to assess the effect of miR-409-3p and miR-216b on TrkB-Shc expression.
RESULTS
miR-409-3p and miR-216b were found to regulate the TrkB-Shc 3'UTR through the identified putative binding sites. When the effect of the miRNAs on TrkB was assessed using SHSY5Y neuronal cells, differential effects were observed between mRNA and protein expression.
CONCLUSION
This study highlights the importance of miRNA-mediated regulation in TrkB signaling.
背景/目的：原肌球蛋白相关激酶B受体（TrkB）介导的信号传导对神经元分化、存活、可塑性和认知至关重要。在本研究中，重点关注TrkB-Shc（一种神经元特异性转录本），以确定微小RNA（miRNA）是否在TrkB-Shc调控中发挥作用。
方法
结合生物信息学和分子基因表达分析技术来评估miR-409-3p和miR-216b对TrkB-Shc表达的影响。
结果
发现miR-409-3p和miR-216b通过已鉴定的假定结合位点调控TrkB-Shc 3'非翻译区。当使用SHSY5Y神经元细胞评估miRNA对TrkB的影响时，在mRNA和蛋白质表达之间观察到了差异效应。
结论
本研究强调了miRNA介导的调控在TrkB信号传导中的重要性。
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