帕金森病大鼠纹状体提取液诱导骨髓间充质干细胞向神经样细胞分化。
Neuronal-like differentiation of bone marrow-derived mesenchymal stem cells induced by striatal extracts from a rat model of Parkinson's disease.
机构信息
Department of Neurology, Xuzhou Central Hospital, Xuzhou 221009, Jiangsu Province, China.
Department of Emergency, Dongying People's Hospital, Dongying 257091, Shandong Province, China.
出版信息
Neural Regen Res. 2012 Dec 5;7(34):2673-80. doi: 10.3969/j.issn.1673-5374.2012.34.004.
A rat model of Parkinson's disease was established by 6-hydroxydopamine injection into the medial forebrain bundle. Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from the femur and tibia, and were co-cultured with 10% and 60% lesioned or intact striatal extracts. The results showed that when exposed to lesioned striatal extracts, BMSCs developed bipolar or multi-polar morphologies, and there was an increase in the percentage of cells that expressed glial fibrillary acidic protein (GFAP), nestin and neuron-specific enolase (NSE). Moreover, the percentage of NSE-positive cells increased with increasing concentrations of lesioned striatal extracts. However, intact striatal extracts only increased the percentage of GFAP-positive cells. The findings suggest that striatal extracts from Parkinson's disease rats induce BMSCs to differentiate into neuronal-like cells in vitro.
通过向内侧前脑束注射 6-羟多巴胺建立帕金森病大鼠模型。从股骨和胫骨中分离出骨髓间充质干细胞（BMSCs），并与 10%和 60%损伤或完整纹状体提取物共培养。结果表明，当暴露于损伤纹状体提取物时，BMSCs 呈现双极或多极形态，胶质纤维酸性蛋白（GFAP）、巢蛋白和神经元特异性烯醇化酶（NSE）表达的细胞百分比增加。此外，NSE 阳性细胞的百分比随着损伤纹状体提取物浓度的增加而增加。然而，完整纹状体提取物仅增加 GFAP 阳性细胞的百分比。这些发现表明，来自帕金森病大鼠的纹状体提取物在体外诱导 BMSCs 分化为神经元样细胞。
Zotero 插件