Department of Neurology, Xuzhou Central Hospital, Xuzhou 221009, Jiangsu Province, China.
Department of Emergency, Dongying People's Hospital, Dongying 257091, Shandong Province, China.
Neural Regen Res. 2012 Dec 5;7(34):2673-80. doi: 10.3969/j.issn.1673-5374.2012.34.004.
A rat model of Parkinson's disease was established by 6-hydroxydopamine injection into the medial forebrain bundle. Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from the femur and tibia, and were co-cultured with 10% and 60% lesioned or intact striatal extracts. The results showed that when exposed to lesioned striatal extracts, BMSCs developed bipolar or multi-polar morphologies, and there was an increase in the percentage of cells that expressed glial fibrillary acidic protein (GFAP), nestin and neuron-specific enolase (NSE). Moreover, the percentage of NSE-positive cells increased with increasing concentrations of lesioned striatal extracts. However, intact striatal extracts only increased the percentage of GFAP-positive cells. The findings suggest that striatal extracts from Parkinson's disease rats induce BMSCs to differentiate into neuronal-like cells in vitro.
通过向内侧前脑束注射 6-羟多巴胺建立帕金森病大鼠模型。从股骨和胫骨中分离出骨髓间充质干细胞（BMSCs），并与 10%和 60%损伤或完整纹状体提取物共培养。结果表明，当暴露于损伤纹状体提取物时，BMSCs 呈现双极或多极形态，胶质纤维酸性蛋白（GFAP）、巢蛋白和神经元特异性烯醇化酶（NSE）表达的细胞百分比增加。此外，NSE 阳性细胞的百分比随着损伤纹状体提取物浓度的增加而增加。然而，完整纹状体提取物仅增加 GFAP 阳性细胞的百分比。这些发现表明，来自帕金森病大鼠的纹状体提取物在体外诱导 BMSCs 分化为神经元样细胞。
