Mojarad Tourandokht Baluchnejad, Roghani Mehrdad
Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Neurophysiology Research Center, Shahed University, Tehran, Iran.
Basic Clin Neurosci. 2014 Spring;5(2):124-30.
Temporal lobe epilepsy (TLE) is a long lasting neurological disorder in which patients suffer from spontaneous seizures. New treatments with novel mechanisms of action are needed to help those patients whose seizures are resistant to available drugs. In this study, we investigated the possible neuroprotective effect of berberine in an intrahippocampal kainate model of TLE in rat.
In the present study, the anticonvulsant and antioxidant effects of intraperitoneal administration of berberine (25, 50 and 100 mg/kg), was evaluated in intrahippocampal kainate (4µg)-induced TLE in rats.
The results showed that the kainate rats exhibit acute and spontaneous seizures in 24 hours and two weeks after intrahippocampal kainic acid injection. Administration of berberine, significantly decreased the Racine score and rate of incidence of seizure in kainate rats (P<0.05). On the other hand, berberine ameliorated the lipid peroxidation (P<0.001) and nitrite (P<0.001) level, but had no effect on SOD activity.
These data suggest that berberine pretreatment could attenuate spontaneous recurrent seizures. Since, administration of berberine decreased lipid peroxidation in kainate rats, it seems that berberine favorable effect is due to its effectiveness in lessening of oxidative stress in rat.
颞叶癫痫（TLE）是一种持续性神经疾病，患者会出现自发性癫痫发作。需要具有新型作用机制的新疗法来帮助那些对现有药物耐药的癫痫患者。在本研究中，我们在大鼠海马内注射红藻氨酸诱发的颞叶癫痫模型中研究了黄连素可能的神经保护作用。
在本研究中，评估了腹腔注射黄连素（25、50和100mg/kg）对大鼠海马内注射红藻氨酸（4μg）诱发的颞叶癫痫的抗惊厥和抗氧化作用。
结果显示，红藻氨酸处理的大鼠在海马内注射红藻氨酸后的24小时和两周内出现急性和自发性癫痫发作。给予黄连素可显著降低红藻氨酸处理大鼠的拉辛评分和癫痫发作发生率（P<0.05）。另一方面，黄连素改善了脂质过氧化水平（P<0.001）和亚硝酸盐水平（P<0.001），但对超氧化物歧化酶活性没有影响。
这些数据表明，黄连素预处理可减轻自发性反复癫痫发作。由于黄连素给药降低了红藻氨酸处理大鼠的脂质过氧化水平，似乎黄连素的有益作用归因于其减轻大鼠氧化应激的有效性。
