Kim Hyun-Jeong, Park Min Jeong, Park Hee-Juhn, Chung Won-Yoon, Kim Ki-Rim, Park Kwang-Kyun
Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, Korea ; Department of Oral Biology, Oral Cancer Research Institute, BK21 PLUS project, Yonsei University College of Dentistry, Seoul, Korea.
Department of Pharmaceutical Engineering, Sangji University, Wonju, Korea.
J Cancer Prev. 2014 Sep;19(3):179-86. doi: 10.15430/JCP.2014.19.3.179.
Allium victorialis var. platyphyllum is an edible perennial herb and has been used as a vegetable or as a Korean traditional medicine. Allium species have received much attention owing to their diverse pharmacological properties, including antioxidative, anti-inflammatory, and anticancer activities. However, A. victorialis var. platyphyllum needs more study.
The chemopreventive potential of A. victorialis var. platyphyllum methanol extracts was examined by measuring 12-O-tetra-decanoylphorbol 13-acetate (TPA)-induced superoxide anion production in the differentiated HL-60 cells, TPA-induced mouse ear edema, and Ames/Salmonella mutagenicity. The apoptosis-inducing capabilities of the extracts were evaluated by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, 4',6-diamidino-2-phenylindole staining, and the DNA fragmentation assay in human colon cancer HT-29 cells. Antimetastatic activities of the extracts were also investigated in an experimental mouse lung metastasis model.
The methanol extracts of A. victorialis var. platyphyllum rhizome (AVP-R) and A. victorialis var. platyphyllum stem (AVP-S) dose-dependently inhibited the TPA-induced generation of superoxide anion in HL-60 cells and TPA-induced ear edema in mice, as well as 7,12-dimethylbenz[a]anthracene (DMBA) and tert-butyl hydroperoxide (t-BOOH) -induced bacterial mutagenesis. AVP-R and AVP-S reduced cell viability in a dose-related manner and induced apoptotic morphological changes and internucleosomal DNA fragmentation in HT-29 cells. In the experimental mouse lung metastasis model, the formation of tumor nodules in lung tissue was significantly inhibited by the treatment of the extracts.
AVP-R and AVP-S possess antioxidative, anti-inflammatory, antimutagenic, proapoptotic, and antimetastatic activities. Therefore, these extracts can serve as a beneficial supplement for the prevention and treatment of cancer.
宽叶山葱是一种可食用的多年生草本植物，已被用作蔬菜或韩国传统药物。葱属植物因其多样的药理特性，包括抗氧化、抗炎和抗癌活性而备受关注。然而，宽叶山葱还需要更多的研究。
通过测量12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）诱导的分化HL - 60细胞中超氧阴离子的产生、TPA诱导的小鼠耳肿胀以及Ames/沙门氏菌致突变性，来检测宽叶山葱甲醇提取物的化学预防潜力。通过3 -（4,5 - 二甲基 - 2 - 噻唑基）- 2,5 - 二苯基 - 2H - 四氮唑溴盐试验、4',6 - 二脒基 - 2 - 苯基吲哚染色以及人结肠癌HT - 29细胞中的DNA片段化试验，评估提取物的诱导凋亡能力。还在实验性小鼠肺转移模型中研究了提取物的抗转移活性。
宽叶山葱根茎甲醇提取物（AVP - R）和宽叶山葱茎甲醇提取物（AVP - S）剂量依赖性地抑制TPA诱导的HL - 60细胞中超氧阴离子的产生、TPA诱导的小鼠耳肿胀，以及7,12 - 二甲基苯并[a]蒽（DMBA）和叔丁基过氧化氢（t - BOOH）诱导的细菌致突变性。AVP - R和AVP - S以剂量相关的方式降低细胞活力，并诱导HT - 29细胞发生凋亡形态变化和核小体间DNA片段化。在实验性小鼠肺转移模型中，提取物处理显著抑制了肺组织中肿瘤结节的形成。
AVP - R和AVP - S具有抗氧化、抗炎、抗诱变、促凋亡和抗转移活性。因此，这些提取物可作为预防和治疗癌症的有益补充剂。
