Rizzello Francesca, De Paolis Angelo, Durante Miriana, Blando Federica, Mita Giovanni, Caretto Sofia
Istituto di Scienze delle Produzioni Alimentari-CNR, Via Prov.le Monteroni, 73100 Lecce, Italy.
Int J Mol Sci. 2014 Oct 21;15(10):19092-105. doi: 10.3390/ijms151019092.
Plant cell cultures as valuable tools for the production of specific metabolites can be greatly improved by the application of elicitors including cyclodextrins (CDs) for enhancing the yields of the desired plant compounds. Here the effects of 2,6-dimethyl-β-cyclodextrins (DIMEB) on the production of carotenoids and quinones from Artemisia annua L. cell suspension cultures were investigated. The addition of 50 mM DIMEB induced an early increase of intracellular carotenoid and quinone contents, which could be observed to a higher extent for lutein (10-fold), Q9 (3-fold) and Q10 (2.5-fold). Real Time PCR analysis revealed that the expression of 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR) gene in DIMEB treated cell cultures after three days was 2.5-fold higher than in untreated samples, thus suggesting that the DIMEB induced increase of carotenoids and quinones could be due to the induction of the plastidial isoprenoid biosynthetic route. In addition, the DIMEB treatment induced an enhanced release of carotenoids and quinones into the culture medium of A. annua cell suspension cultures possibly due to the ability of CDs to form inclusion complexes with hydrophobic molecules.
植物细胞培养作为生产特定代谢产物的宝贵工具，可通过应用包括环糊精（CDs）在内的诱导子来显著提高所需植物化合物的产量。在此，研究了2,6 - 二甲基 - β - 环糊精（DIMEB）对黄花蒿细胞悬浮培养物中类胡萝卜素和醌类物质产生的影响。添加50 mM DIMEB可诱导细胞内类胡萝卜素和醌类物质含量早期增加，叶黄素（10倍）、Q9（3倍）和Q10（2.5倍）的增加更为明显。实时定量PCR分析显示，处理三天后，DIMEB处理的细胞培养物中1 - 脱氧 - d - 木酮糖 - 5 - 磷酸还原异构酶（DXR）基因的表达比未处理样品高2.5倍，这表明DIMEB诱导的类胡萝卜素和醌类物质增加可能是由于质体类异戊二烯生物合成途径的诱导。此外，DIMEB处理导致黄花蒿细胞悬浮培养物培养基中类胡萝卜素和醌类物质的释放增加，这可能是由于CDs与疏水分子形成包合物的能力。
