Zamboni Anita, Gatto Pamela, Cestaro Alessandro, Pilati Stefania, Viola Roberto, Mattivi Fulvio, Moser Claudio, Velasco Riccardo
IASMA Research and Innovation Center, Fondazione Edmund Mach, S. Michele a/Adige I-38010, Italy.
BMC Genomics. 2009 Aug 6;10:363. doi: 10.1186/1471-2164-10-363.
In response to pathogen attack, grapevine synthesizes phytoalexins belonging to the family of stilbenes. Grapevine cell cultures represent a good model system for studying the basic mechanisms of plant response to biotic and abiotic elicitors. Among these, modified beta-cyclodextrins seem to act as true elicitors inducing strong production of the stilbene resveratrol.
The transcriptome changes of Vitis riparia x Vitis berlandieri grapevine cells in response to the modified beta-cyclodextrin, DIMEB, were analyzed 2 and 6 h after treatment using a suppression subtractive hybridization experiment and a microarray analysis respectively. At both time points, we identified a specific set of induced genes belonging to the general phenylpropanoid metabolism, including stilbenes and hydroxycinnamates, and to defence proteins such as PR proteins and chitinases. At 6 h we also observed a down-regulation of the genes involved in cell division and cell-wall loosening.
We report the first large-scale study of the molecular effects of DIMEB, a resveratrol inducer, on grapevine cell cultures. This molecule seems to mimic a defence elicitor which enhances the physical barriers of the cell, stops cell division and induces phytoalexin synthesis.
为应对病原体攻击，葡萄会合成属于芪类家族的植物抗毒素。葡萄细胞培养物是研究植物对生物和非生物诱导子反应基本机制的良好模型系统。其中，修饰的β-环糊精似乎可作为真正的诱导子，诱导强烈产生芪类白藜芦醇。
分别使用抑制性消减杂交实验和微阵列分析，在处理后2小时和6小时分析河岸葡萄×沙地葡萄细胞对修饰的β-环糊精DIMEB的转录组变化。在两个时间点，我们都鉴定出一组特定的诱导基因，这些基因属于一般的苯丙烷类代谢，包括芪类和羟基肉桂酸类，以及防御蛋白，如病程相关蛋白（PR蛋白）和几丁质酶。在6小时时，我们还观察到参与细胞分裂和细胞壁松弛的基因下调。
我们报道了首个关于白藜芦醇诱导剂DIMEB对葡萄细胞培养物分子效应的大规模研究。该分子似乎模拟了一种防御诱导子，可增强细胞的物理屏障、停止细胞分裂并诱导植物抗毒素合成。
