利用序列相关扩增多态性标记对430份中国狗牙根种质进行遗传分析。

Genetic analysis of 430 Chinese Cynodon dactylon accessions using sequence-related amplified polymorphism markers.

作者信息

Huang Chunqiong, Liu Guodao, Bai Changjun, Wang Wenqiang

机构信息

Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Danzhou 571737, China.

出版信息

Int J Mol Sci. 2014 Oct 21;15(10):19134-46. doi: 10.3390/ijms151019134.

DOI:10.3390/ijms151019134

PMID:25338051

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4227265/

Abstract

Although Cynodon dactylon (C. dactylon) is widely distributed in China, information on its genetic diversity within the germplasm pool is limited. The objective of this study was to reveal the genetic variation and relationships of 430 C. dactylon accessions collected from 22 Chinese provinces using sequence-related amplified polymorphism (SRAP) markers. Fifteen primer pairs were used to amplify specific C. dactylon genomic sequences. A total of 481 SRAP fragments were generated, with fragment sizes ranging from 260-1800 base pairs (bp). Genetic similarity coefficients (GSC) among the 430 accessions averaged 0.72 and ranged from 0.53-0.96. Cluster analysis conducted by two methods, namely the unweighted pair-group method with arithmetic averages (UPGMA) and principle coordinate analysis (PCoA), separated the accessions into eight distinct groups. Our findings verify that Chinese C. dactylon germplasms have rich genetic diversity, which is an excellent basis for C. dactylon breeding for new cultivars.

摘要

虽然狗牙根在中国分布广泛，但关于其种质库内遗传多样性的信息有限。本研究的目的是利用序列相关扩增多态性（SRAP）标记揭示从中国22个省份收集的430份狗牙根种质的遗传变异和关系。使用15对引物对扩增狗牙根特定基因组序列。共产生了481个SRAP片段，片段大小范围为260 - 1800碱基对（bp）。430份种质之间的遗传相似系数（GSC）平均为0.72，范围为0.53 - 0.96。通过两种方法进行聚类分析，即算术平均数的非加权成组配对法（UPGMA）和主坐标分析（PCoA），将这些种质分为八个不同的组。我们的研究结果证实中国狗牙根种质具有丰富的遗传多样性，这是狗牙根新品种育种的良好基础。